Cloned (Comment) | Organism |
---|---|
expression in Escherichia DH5 alpha, phylogentic analyses | Talaromyces purpureogenus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.098 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Talaromyces purpureogenus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Talaromyces purpureogenus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
monomer, SDS-PAGE | Talaromyces purpureogenus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces purpureogenus | A4UU45 | ATCC strain MYA-38 | - |
Purification (Comment) | Organism |
---|---|
precipitation, chromatography in a DEAESephadex A 25, cation exchange chromatography in SP Sepharose | Talaromyces purpureogenus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Talaromyces purpureogenus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
p-nitrophenyl-alpha-L-arabinopyranoside, 0.4% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
0.011 | - |
p-nitrophenyl-beta-D-xylopyranoside, 0.9% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
0.033 | - |
p-nitrophenyl-beta-D-mannopyranoside, 2.8% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
0.06 | - |
p-nitrophenyl-alpha-D-glucopyranoside, 5.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
0.073 | - |
p-nitrophenyl-alpha-D-galactopyranoside, 6.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
0.1 | - |
p-nitrophenyl-beta-D-fucopyranoside, 8.3% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
0.11 | - |
p-nitrophenyl-beta-D-glucopyranoside, 9.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
0.23 | - |
p-nitrophenyl-beta-D-galactopyranoside, 19% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
1.2 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ABF 2 shows weak activity on natural polymeric substrates, such as sugar beet arabinan, debranched arabinan, and arabinoxylan, the enzyme is highly specific for alpha-L-arabinofuranosyl linkages, although some activity (20%) is observed with p-nitrophenyl-beta-D-galactopyranoside | Talaromyces purpureogenus | ? | - |
? | |
p-nitrophenyl-alpha-D-galactopyranoside + H2O | 6.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus | p-nitrophenol + alpha-D-galactopyranose | - |
? | |
p-nitrophenyl-alpha-D-glucopyranoside + H2O | 5.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus | p-nitrophenol + alpha-D-glucopyranose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Talaromyces purpureogenus | p-nitrophenol + L-arabinofuranose | - |
? | |
p-nitrophenyl-alpha-L-arabinopyranoside + H2O | 0.4% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus | p-nitrophenol + L-arabinopyranose | - |
? | |
p-nitrophenyl-beta-D-fucopyranoside + H2O | 8.3% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus | p-nitrophenol + beta-D-fucopyranose | - |
? | |
p-nitrophenyl-beta-D-galactopyranoside + H2O | 19% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus | p-nitrophenol + beta-D-galactopyranose | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside + H2O | 9.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus | p-nitrophenol + ? | - |
? | |
p-nitrophenyl-beta-D-mannopyranoside + H2O | 2.8% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus | p-nitrophenol + beta-D-mannopyranose | - |
? | |
p-nitrophenyl-beta-D-xylopyranoside + H2O | 0.9% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside | Talaromyces purpureogenus | p-nitrophenol + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Talaromyces purpureogenus |
Synonyms | Comment | Organism |
---|---|---|
ABF 2 | - |
Talaromyces purpureogenus |
alpha-L-arabinofuranosidase | - |
Talaromyces purpureogenus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Talaromyces purpureogenus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 70 | - |
Talaromyces purpureogenus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
ABF 2, when incubated at 50°C, retains over 50% of its activity after 2 h | Talaromyces purpureogenus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.45 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Talaromyces purpureogenus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Talaromyces purpureogenus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.6 | 6.6 | - |
Talaromyces purpureogenus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Talaromyces purpureogenus | isoelectrofocusing | - |
5.3 |