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Literature summary for 3.2.1.55 extracted from

  • Fritz, M.; Ravanal, M.C.; Braet, C.; Eyzaguirre, J.
    A family 51 alpha-l-arabinofuranosidase from Penicillium purpurogenum: purification, properties and amino acid sequence (2008), Mycol. Res., 112, 933-942.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia DH5 alpha, phylogentic analyses Talaromyces purpureogenus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.098
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Talaromyces purpureogenus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Talaromyces purpureogenus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
monomer, SDS-PAGE Talaromyces purpureogenus

Organism

Organism UniProt Comment Textmining
Talaromyces purpureogenus A4UU45 ATCC strain MYA-38
-

Purification (Commentary)

Purification (Comment) Organism
precipitation, chromatography in a DEAESephadex A 25, cation exchange chromatography in SP Sepharose Talaromyces purpureogenus

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Talaromyces purpureogenus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
p-nitrophenyl-alpha-L-arabinopyranoside, 0.4% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus
0.011
-
p-nitrophenyl-beta-D-xylopyranoside, 0.9% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus
0.033
-
p-nitrophenyl-beta-D-mannopyranoside, 2.8% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus
0.06
-
p-nitrophenyl-alpha-D-glucopyranoside, 5.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus
0.073
-
p-nitrophenyl-alpha-D-galactopyranoside, 6.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus
0.1
-
p-nitrophenyl-beta-D-fucopyranoside, 8.3% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus
0.11
-
p-nitrophenyl-beta-D-glucopyranoside, 9.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus
0.23
-
p-nitrophenyl-beta-D-galactopyranoside, 19% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus
1.2
-
p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ABF 2 shows weak activity on natural polymeric substrates, such as sugar beet arabinan, debranched arabinan, and arabinoxylan, the enzyme is highly specific for alpha-L-arabinofuranosyl linkages, although some activity (20%) is observed with p-nitrophenyl-beta-D-galactopyranoside Talaromyces purpureogenus ?
-
?
p-nitrophenyl-alpha-D-galactopyranoside + H2O 6.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus p-nitrophenol + alpha-D-galactopyranose
-
?
p-nitrophenyl-alpha-D-glucopyranoside + H2O 5.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus p-nitrophenol + alpha-D-glucopyranose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Talaromyces purpureogenus p-nitrophenol + L-arabinofuranose
-
?
p-nitrophenyl-alpha-L-arabinopyranoside + H2O 0.4% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus p-nitrophenol + L-arabinopyranose
-
?
p-nitrophenyl-beta-D-fucopyranoside + H2O 8.3% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus p-nitrophenol + beta-D-fucopyranose
-
?
p-nitrophenyl-beta-D-galactopyranoside + H2O 19% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus p-nitrophenol + beta-D-galactopyranose
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O 9.1% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus p-nitrophenol + ?
-
?
p-nitrophenyl-beta-D-mannopyranoside + H2O 2.8% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus p-nitrophenol + beta-D-mannopyranose
-
?
p-nitrophenyl-beta-D-xylopyranoside + H2O 0.9% of the activity of p-nitrophenyl-alpha-L-arabinofuranoside Talaromyces purpureogenus p-nitrophenol + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Talaromyces purpureogenus

Synonyms

Synonyms Comment Organism
ABF 2
-
Talaromyces purpureogenus
alpha-L-arabinofuranosidase
-
Talaromyces purpureogenus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Talaromyces purpureogenus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 70
-
Talaromyces purpureogenus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
ABF 2, when incubated at 50°C, retains over 50% of its activity after 2 h Talaromyces purpureogenus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.45
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Talaromyces purpureogenus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Talaromyces purpureogenus

pH Range

pH Minimum pH Maximum Comment Organism
2.6 6.6
-
Talaromyces purpureogenus

pI Value

Organism Comment pI Value Maximum pI Value
Talaromyces purpureogenus isoelectrofocusing
-
5.3