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Literature summary for 3.2.1.55 extracted from
- Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation (2007), Extremophiles, 11, 123-132.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
4 * 82000, SDS-PAGE | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharolobus solfataricus | specifically induced by xylan and repressed by monosaccharides like D-glucose and L-arabinose | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus P2 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.63 | - |
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-glucopyranoside + H2O | 1.7% of activity with p-nitrophenyl-alpha-L-arabinofuranoside | Saccharolobus solfataricus | 2-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | 9% of activity with p-nitrophenyl-alpha-L-arabinofuranoside | Saccharolobus solfataricus | 4-nitrophenol + D-glucopyranose | - |
? | |
| additional information | specifically induced by xylan and repressed by monosaccharides like D-glucose and L-arabinose | Saccharolobus solfataricus | ? | - |
? | |
| o-nitrophenyl-beta-D-xylopyranoside + H2O | 3% of activity with p-nitrophenyl-alpha-L-arabinofuranoside | Saccharolobus solfataricus | o-nitrophenol + beta-D-xylopyranose | - |
? | |
| p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Saccharolobus solfataricus | p-nitrophenol + L-arabinofuranose | - |
? | |
| p-nitrophenyl-alpha-L-arabinopyranoside + H2O | 46% of activity with p-nitrophenyl-alpha-L-arabinofuranoside | Saccharolobus solfataricus | p-nitrophenol + alpha-L-arabinopyranose | - |
? | |
| p-nitrophenyl-beta-D-xylopyranoside + H2O | 14% of activity with p-nitrophenyl-alpha-L-arabinofuranoside | Saccharolobus solfataricus | p-nitrophenol + beta-D-xylopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 82000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-D-xylosidase/alpha-L-arabinosidase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
24 h, stable | Saccharolobus solfataricus |
| 90 | - |
half-life: 2 h | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Saccharolobus solfataricus |
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Release 2023.1 (January 2023)
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