Literature summary for 3.2.1.55 extracted from

Jordan, D.B.; Li, X.
Variation in relative substrate specificity of bifunctional beta -D-xylosidase/alpha-L-arabinofuranosidase by single-site mutations: Roles of substrate distortion and recognition (2007), Biochim. Biophys. Acta, 1774, 1192-1198.

Search for more literature for 3.2.1.55

Protein Variants

Protein Variants	Comment	Organism
D127A	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 191860fold lower than wild-type value	Selenomonas ruminantium
D14A	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 2727fold lower than wild-type value	Selenomonas ruminantium
E186A	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 18333fold lower than wild-type value	Selenomonas ruminantium
F31A	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 9.5fold lower than wild-type value	Selenomonas ruminantium
F508A	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 18fold lower than wild-type value	Selenomonas ruminantium
H248A	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 334fold lower than wild-type value	Selenomonas ruminantium
R290A	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 1765fold lower than wild-type value	Selenomonas ruminantium
R290G	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 3028fold lower than wild-type value	Selenomonas ruminantium
R290H	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 1089fold lower than wild-type value	Selenomonas ruminantium
R290K	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 314fold lower than wild-type value	Selenomonas ruminantium
R290Q	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is213fold lower than wild-type value	Selenomonas ruminantium
R290V	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 1709fold lower than wild-type value	Selenomonas ruminantium
W73A	kcat/Km for p-nitrophenyl-alpha-L-arabinofuranoside is 2520fold lower than wild-type value	Selenomonas ruminantium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.771	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme D14A	Selenomonas ruminantium
0.779	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme D127A	Selenomonas ruminantium
0.81	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, wild-type enzyme	Selenomonas ruminantium
1.33	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme E186A	Selenomonas ruminantium
5.1	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290G	Selenomonas ruminantium
5.82	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme F508A	Selenomonas ruminantium
7.97	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290A	Selenomonas ruminantium
9.07	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290V	Selenomonas ruminantium
17.7	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290Q	Selenomonas ruminantium
26.8	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme F31A	Selenomonas ruminantium
40.5	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290K	Selenomonas ruminantium
52.1	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290H	Selenomonas ruminantium

Organism

Organism	UniProt	Comment	Textmining
Selenomonas ruminantium	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl-alpha-L-arabinofuranoside + H2O	-	Selenomonas ruminantium	p-nitrophenol + L-arabinofuranose	-	?

Synonyms

Synonyms	Comment	Organism
beta-D-xylosidase/alpha-L-arabinofuranosidase	bifunctional enzyme displaying activity of EC 3.2.1.37 and EC 3.2.1.55	Selenomonas ruminantium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0000134	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme D127A	Selenomonas ruminantium
0.000239	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme E186A	Selenomonas ruminantium
0.000936	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme D14A	Selenomonas ruminantium
0.00553	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290G	Selenomonas ruminantium
0.0149	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290A	Selenomonas ruminantium
0.0175	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290V	Selenomonas ruminantium
0.158	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290H	Selenomonas ruminantium
0.275	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290Q	Selenomonas ruminantium
0.426	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme R290K	Selenomonas ruminantium
1.09	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme F508A	Selenomonas ruminantium
2.68	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, wild-type enzyme	Selenomonas ruminantium
9.33	-	p-nitrophenyl-alpha-L-arabinofuranoside	pH 5.3, 25°C, mutant enzyme F31A	Selenomonas ruminantium

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)