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Literature summary for 3.2.1.55 extracted from

  • Wan, C.F.; Chen, W.H.; Chen, C.T.; Chang, M.D.; Lo, L.C.; Li, Y.K.
    Mutagenesis and mechanistic study of a glycoside hydrolase family 54 alpha-L-arabinofuranosidase from Trichoderma koningii (2007), Biochem. J., 401, 551-558.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Trichoderma koningii

Protein Variants

Protein Variants Comment Organism
D170N kcat is 31fold lower than wild-type value Trichoderma koningii
D221N significant decrease in catalytic activity, kcat is 7000fold lower than wild-type value Trichoderma koningii
D299G kcat is 263fold lower than wild-type value Trichoderma koningii
D299N significant decrease in catalytic activity, kcat is 1300fold lower than wild-type value Trichoderma koningii
E223Q enzyme is nearly inactive Trichoderma koningii
E310G kcat is 70fold lower than wild-type value Trichoderma koningii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme D299N Trichoderma koningii
0.1
-
2,5-dinitrophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.2
-
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.21
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme D299G Trichoderma koningii
0.21
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.22
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme D221N Trichoderma koningii
0.28
-
phenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
0.28
-
2,5-dinitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
0.3
-
p-cyanophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
0.3
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme D170N Trichoderma koningii
0.31
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
0.32
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, wild-type enzyme Trichoderma koningii
0.37
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme E223G Trichoderma koningii
0.42
-
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
0.54
-
m-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
0.65
-
m-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.69
-
p-cyanophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.88
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme E223Q Trichoderma koningii
3
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme E310G Trichoderma koningii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Trichoderma koningii

Organism

Organism UniProt Comment Textmining
Trichoderma koningii
-
-
-
Trichoderma koningii G-39
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Trichoderma koningii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii 2,5-dinitrophenol + L-arabinofuranose
-
?
2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii G-39 2,5-dinitrophenol + L-arabinofuranose
-
?
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii 4-chloro-2-nitrophenol + L-arabinofuranose
-
?
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii G-39 4-chloro-2-nitrophenol + L-arabinofuranose
-
?
m-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii m-nitrophenol + L-arabinofuranose
-
?
m-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii G-39 m-nitrophenol + L-arabinofuranose
-
?
p-cyanophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii p-cyanophenol + L-arabinofuranose
-
?
p-cyanophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii G-39 p-cyanophenol + L-arabinofuranose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii p-nitrophenol + L-arabinofuranose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii G-39 p-nitrophenol + L-arabinofuranose
-
?
phenyl-alpha-L-arabinofuranoside + H2O
-
Trichoderma koningii phenol + L-arabinofuranose
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Trichoderma koningii

Synonyms

Synonyms Comment Organism
ABF
-
Trichoderma koningii
GH family 54 alpha-L-arabinofuranosidase
-
Trichoderma koningii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
m-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.0004
-
p-cyanophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.0005
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme E223Q Trichoderma koningii
0.003
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme D221N Trichoderma koningii
0.014
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.016
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme D299N Trichoderma koningii
0.03 0.55 4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
0.08
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme D299G Trichoderma koningii
0.12
-
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
0.3
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme E310G Trichoderma koningii
0.68
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme D170N Trichoderma koningii
0.69
-
2,5-dinitrophenyl-alpha-L-arabinofuranoside pH 6.5, mutant enzyme D299G Trichoderma koningii
1.23
-
phenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
1.7
-
p-cyanophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
2.02
-
m-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
2.84
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
6.52
-
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
8.25
-
2,5-dinitrophenyl-alpha-L-arabinofuranoside pH 6.5, wild-type enzyme Trichoderma koningii
21
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, wild-type enzyme Trichoderma koningii
22
-
p-nitrophenyl-alpha-L-arabinofuranoside pH 4.1, 25°C, mutant enzyme E223G Trichoderma koningii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8 3.2
-
Trichoderma koningii

pH Range

pH Minimum pH Maximum Comment Organism
2 4 pH 2: about 70% of maximal activity, pH 4.0: about 80% of maximal activity Trichoderma koningii

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 8.3 25°C, 2 h, stable Trichoderma koningii