Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Trichoderma koningii |
Protein Variants | Comment | Organism |
---|---|---|
D170N | kcat is 31fold lower than wild-type value | Trichoderma koningii |
D221N | significant decrease in catalytic activity, kcat is 7000fold lower than wild-type value | Trichoderma koningii |
D299G | kcat is 263fold lower than wild-type value | Trichoderma koningii |
D299N | significant decrease in catalytic activity, kcat is 1300fold lower than wild-type value | Trichoderma koningii |
E223Q | enzyme is nearly inactive | Trichoderma koningii |
E310G | kcat is 70fold lower than wild-type value | Trichoderma koningii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme D299N | Trichoderma koningii | |
0.1 | - |
2,5-dinitrophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.2 | - |
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.21 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme D299G | Trichoderma koningii | |
0.21 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.22 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme D221N | Trichoderma koningii | |
0.28 | - |
phenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
0.28 | - |
2,5-dinitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
0.3 | - |
p-cyanophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
0.3 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme D170N | Trichoderma koningii | |
0.31 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
0.32 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, wild-type enzyme | Trichoderma koningii | |
0.37 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme E223G | Trichoderma koningii | |
0.42 | - |
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
0.54 | - |
m-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
0.65 | - |
m-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.69 | - |
p-cyanophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.88 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme E223Q | Trichoderma koningii | |
3 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme E310G | Trichoderma koningii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Trichoderma koningii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma koningii | - |
- |
- |
Trichoderma koningii G-39 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Trichoderma koningii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii | 2,5-dinitrophenol + L-arabinofuranose | - |
? | |
2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii G-39 | 2,5-dinitrophenol + L-arabinofuranose | - |
? | |
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii | 4-chloro-2-nitrophenol + L-arabinofuranose | - |
? | |
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii G-39 | 4-chloro-2-nitrophenol + L-arabinofuranose | - |
? | |
m-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii | m-nitrophenol + L-arabinofuranose | - |
? | |
m-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii G-39 | m-nitrophenol + L-arabinofuranose | - |
? | |
p-cyanophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii | p-cyanophenol + L-arabinofuranose | - |
? | |
p-cyanophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii G-39 | p-cyanophenol + L-arabinofuranose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii | p-nitrophenol + L-arabinofuranose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii G-39 | p-nitrophenol + L-arabinofuranose | - |
? | |
phenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma koningii | phenol + L-arabinofuranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Trichoderma koningii |
Synonyms | Comment | Organism |
---|---|---|
ABF | - |
Trichoderma koningii |
GH family 54 alpha-L-arabinofuranosidase | - |
Trichoderma koningii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
m-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.0004 | - |
p-cyanophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.0005 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme E223Q | Trichoderma koningii | |
0.003 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme D221N | Trichoderma koningii | |
0.014 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.016 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme D299N | Trichoderma koningii | |
0.03 | 0.55 | 4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
0.08 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme D299G | Trichoderma koningii | |
0.12 | - |
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
0.3 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme E310G | Trichoderma koningii | |
0.68 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme D170N | Trichoderma koningii | |
0.69 | - |
2,5-dinitrophenyl-alpha-L-arabinofuranoside | pH 6.5, mutant enzyme D299G | Trichoderma koningii | |
1.23 | - |
phenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
1.7 | - |
p-cyanophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
2.02 | - |
m-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
2.84 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
6.52 | - |
4-chloro-2-nitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
8.25 | - |
2,5-dinitrophenyl-alpha-L-arabinofuranoside | pH 6.5, wild-type enzyme | Trichoderma koningii | |
21 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, wild-type enzyme | Trichoderma koningii | |
22 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | pH 4.1, 25°C, mutant enzyme E223G | Trichoderma koningii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.8 | 3.2 | - |
Trichoderma koningii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 4 | pH 2: about 70% of maximal activity, pH 4.0: about 80% of maximal activity | Trichoderma koningii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 8.3 | 25°C, 2 h, stable | Trichoderma koningii |