Literature summary for 3.2.1.55 extracted from

Degrassi, G.; Vindigni, A.; Venturi, V.
A thermostable alpha-arabinofuranosidase from xylanolytic Bacillus pumilus: purification and characterization (2003), J. Biotechnol., 101, 69-79.

Search for more literature for 3.2.1.55

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7	-	4-nitrophenyl-alpha-L-arabinofuranoside	pH 7.0, 37°C	Bacillus pumilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	secretion to the cell culture medium containing arabinogalactan or oat meal	Bacillus pumilus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
220000	-	gel filtration	Bacillus pumilus

Organism

Organism	UniProt	Comment	Textmining
Bacillus pumilus	-	xylanolytic bacterium, strain PS213	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity from cell culture supernatant	Bacillus pumilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O	-	Bacillus pumilus	4-nitrophenol + alpha-L-arabinofuranose	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 56000-60000, SDS-PAGE	Bacillus pumilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Bacillus pumilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	loss of 80% activity after 2 h	Bacillus pumilus
75	-	50% loss of activity after 135 min	Bacillus pumilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Bacillus pumilus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Bacillus pumilus	-	-	4.9

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Release 2023.1 (January 2023)