Literature summary for 3.2.1.55 extracted from

H�vel, K.; Shallom, D.; Niefind, K.; Belakhov, V.; Shoham, G.; Baasov, T.; Shoham, Y.; Schomburg, D.
Crystal structure and snapshots alaong the reaction pathway of a family 51 alpha-L-arabinofuranosidase (2003), EMBO J., 22, 4922-4932.

Crystallization (Commentary)

Crystallization (Comment)	Organism
native and selenomethionine-labeled wild-type enzyme, and mutant E175A in complex with substrates arabinofuranose, Ara-alpha(1-3)-Xyl and 4-nitrophenyl-Ara, from 23% w/v PEG 3350, 0.2 M NH4F, 5% v/v 2-propanol, 0.1 M Tris-HCl, pH 8.0, complex formation by soaking of crystals at 4°C in a cryo-solution containing additionally 12% glycerol and 20 mM of the substrate compounds, X-ray diffraction structure determination and analysis at 1.2-2.1 A resolution, modeling	Geobacillus stearothermophilus

Crystallization (Comment)

Organism

native and selenomethionine-labeled wild-type enzyme, and mutant E175A in complex with substrates arabinofuranose, Ara-alpha(1-3)-Xyl and 4-nitrophenyl-Ara, from 23% w/v PEG 3350, 0.2 M NH4F, 5% v/v 2-propanol, 0.1 M Tris-HCl, pH 8.0, complex formation by soaking of crystals at 4°C in a cryo-solution containing additionally 12% glycerol and 20 mM of the substrate compounds, X-ray diffraction structure determination and analysis at 1.2-2.1 A resolution, modeling

Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	Q9XBQ3	-	-
Geobacillus stearothermophilus T-6	Q9XBQ3	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
1,5-alpha-L-arabinofuranohexaose + 5 H2O = 6 alpha-L-arabinofuranose	catalytic mechanism, 2 stable states in the double displacement mechanism with formation of a transient covalent arabinofuranosyl-enzyme intermediate, structure of enzyme-substrate complex, substrate binding and specificity	Geobacillus stearothermophilus	a

Subunits

Subunits	Comment	Organism
hexamer	-	Geobacillus stearothermophilus
More	each subunit is composed of 2 domains: a (beta/alpha)8-barrel and a 12-stranded beta sandwich with jelly-roll topology	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
alpha-L-arabinofuranosidase	-	Geobacillus stearothermophilus
More	enzyme belongs to glycosidase family 51	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)