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Literature summary for 3.2.1.55 extracted from

  • Sakamoto, T.; Kawasaki, H.
    Purification and properties of two type-B alpha-L-arabinofuranosidases produced by Penicillium chrysogenum (2003), Biochim. Biophys. Acta, 1621, 204-210.
    View publication on PubMed
Search for more literature for 3.2.1.55

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 92% inhibition of AFQ1, 89% inhibition of AFS, at 1 mM Penicillium chrysogenum
Cd2+ 37% inhibition of AFQ1, 15% inhibition of AFS, at 1 mM Penicillium chrysogenum
Cu2+ 76% inhibition of AFQ1, 36% inhibition of AFS, at 1 mM Penicillium chrysogenum
Hg2+ 90% inhibition of AFQ1, 74% inhibition of AFS, at 1 mM Penicillium chrysogenum
L-arabinose 10 mg/ml, inhibition of enzyme AFS1, not AFQ1 Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.86
-
 4-nitrophenyl-alpha-L-arabinofuranoside enzyme AFQ1, pH 5.0, 37°C Penicillium chrysogenum
1.32
-
 4-nitrophenyl-alpha-L-arabinofuranoside enzyme AFS1, pH 5.0, 37°C Penicillium chrysogenum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium chrysogenum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 type B enzyme AFS1, gel filtration Penicillium chrysogenum
52000
-
 x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 Penicillium chrysogenum
71000
-
 type B enzyme AFQ1, gel filtration Penicillium chrysogenum
79000
-
 x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
 strain 31B, 2 distinct type B enzymes encoded by different genes
-
Penicillium chrysogenum 31B
-
 strain 31B, 2 distinct type B enzymes encoded by different genes
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme AFQ1 is a glycoprotein, while enzyme AFS1 is not Penicillium chrysogenum
no glycoprotein AFS1 Penicillium chrysogenum

Purification (Commentary)

Purification (Comment) Organism
both type B enzymes AFQ1, 17fold, and AFS1, 39.7fold, from culture filtrate Penicillium chrysogenum

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.8
-
 purified enzyme AFQ1 Penicillium chrysogenum
13.5
-
 purified enzyme AFS1 Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Penicillium chrysogenum 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Penicillium chrysogenum 31B 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity only of enzyme AFS1, not AFQ1 Penicillium chrysogenum 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity only of enzyme AFS1, not AFQ1 Penicillium chrysogenum 31B 4-nitrophenol + beta-D-xylopyranose
-
 ?
arabino-oligosaccharides + H2O
-
 Penicillium chrysogenum alpha-L-arabinose
-
 ?
arabino-oligosaccharides + H2O
-
 Penicillium chrysogenum 31B alpha-L-arabinose
-
 ?
arabinogalactan + H2O from soybean Penicillium chrysogenum alpha-L-arabinose + ?
-
 ?
arabinogalactan + H2O from soybean Penicillium chrysogenum 31B alpha-L-arabinose + ?
-
 ?
arabinoxylan + H2O from wheat Penicillium chrysogenum alpha-L-arabinose + ?
-
 ?
branched L-arabinan + H2O from sugar beet Penicillium chrysogenum L-arabinose + ?
-
 ?
debranched arabinan + H2O from sugar beet Penicillium chrysogenum alpha-L-arabinose + ?
-
 ?
additional information both type B enzymes show broad substrate specificity, synergistic action between AFQ1, AFS1, and exo-arabinanase in degradation of arabinan Penicillium chrysogenum ?
-
 ?
additional information both type B enzymes show broad substrate specificity, synergistic action between AFQ1, AFS1, and exo-arabinanase in degradation of arabinan Penicillium chrysogenum 31B ?
-
 ?

Subunits

Subunits Comment Organism
? x * 79000, type B enzyme AFQ1, x * 52000, type B enzyme AFS1 Penicillium chrysogenum

Synonyms

Synonyms Comment Organism
AF
-
 Penicillium chrysogenum
AFQ
-
 Penicillium chrysogenum
AFS
-
 Penicillium chrysogenum
alpha-L-arabinofuranosidase
-
 Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Penicillium chrysogenum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Penicillium chrysogenum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.3
-
 4-nitrophenyl-alpha-L-arabinofuranoside enzyme AFQ1, pH 5.0, 37°C Penicillium chrysogenum
59.3
-
 4-nitrophenyl-alpha-L-arabinofuranoside enzyme AFS1, pH 5.0, 37°C Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3 5 enzyme AFS1 Penicillium chrysogenum
4 6.5 enzyme AFQ1 Penicillium chrysogenum