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Literature summary for 3.2.1.55 extracted from

  • Beylot, M.H.; McKie, V.A.; Voragen, A.G.J.; Doeswijk-Voragen, C.H.L.; Gilbert, H.J.
    The Pseudomonas cellulosa glycoside hydrolase family 51 arabinofuranosidase exhibits wide substrate specificity (2001), Biochem. J., 358, 607-614.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.55

Cloned(Commentary)

Cloned (Comment) Organism
gene abf51A amplified by PCR Cellvibrio japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 p-nitrophenyl-alpha-L-arabinofuranoside mutant E194A Cellvibrio japonicus
0.18
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Cellvibrio japonicus
0.26
-
 4-methylumbelliferyl-alpha-L-arabinofuranoside
-
 Cellvibrio japonicus
0.27
-
 wheat arabinoxylan
-
 Cellvibrio japonicus
0.33
-
 sugar beet arabinan
-
 Cellvibrio japonicus
1.58
-
 arabinobiose
-
 Cellvibrio japonicus
1.7
-
 arabinobiose
-
 Cellvibrio japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Cellvibrio japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Streptomyces chartreusis
-
 L-arabinose + ?
-
 ir
beet arabinan + H2O Cellvibrio japonicus
-
 L-arabinose + ?
-
 ir
wheat L-arabino-D-xylan + H2O Cellvibrio japonicus
-
 L-arabinose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bifidobacterium adolescentis
-
-
-
Cellvibrio japonicus
-
-
-
Pseudomonas fluorescens
-
-
-
Streptomyces chartreusis
-
 GS901
-
Streptomyces chartreusis GS901
-
 GS901
-
Thermoclostridium stercorarium O08457
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-alpha-L-arabinobiose + H2O
-
 Cellvibrio japonicus L-arabinose
-
 ?
1,5-alpha-L-arabinofuranohexaose + H2O
-
 Cellvibrio japonicus L-arabinose + L-arabinopentaose
-
 ?
1,5-alpha-L-arabinotetraose + H2O
-
 Cellvibrio japonicus L-arabinose
-
 ?
1,5-alpha-L-arabinotriose + 2 H2O
-
 Cellvibrio japonicus 3 L-arabinose
-
 ?
2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis alpha-L-arabinofuranose
-
 ?
2-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis GS901 alpha-L-arabinofuranose
-
 ?
3-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis alpha-L-arabinofuranose
-
 ?
5-O-alpha-L-arabinofuranosyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis alpha-L-arabinofuranose
-
 ?
alpha-L-arabinofuranosyl 4-methylumbelliferone + H2O
-
 Cellvibrio japonicus methylumbelliferone + alpha-L-arabinofuranose
-
 ?
beet arabinan + H2O
-
 Streptomyces chartreusis L-arabinose + ?
-
 ir
beet arabinan + H2O
-
 Cellvibrio japonicus L-arabinose + ?
-
 ir
L-arabinan + H2O
-
 Streptomyces chartreusis (1,5-alpha-L-arabinose)25 + ?
-
 ?
L-arabinan + H2O preferentially removes alpha 1,2 and alpha 1,3-linked arabinofuranose side chains Cellvibrio japonicus (1,5-alpha-L-arabinose)25 + ?
-
 ?
L-arabinan + H2O
-
 Streptomyces chartreusis GS901 (1,5-alpha-L-arabinose)25 + ?
-
 ?
L-arabinopentaose + H2O
-
 Cellvibrio japonicus L-arabinotetraose + L-arabinose
-
 ?
L-arabinoxylan + H2O
-
 Bifidobacterium adolescentis L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Streptomyces chartreusis L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Thermoclostridium stercorarium L-arabinose + ?
-
 ir
L-arabinoxylan + H2O preferentially removes alpha-1,2- and alpha-1,3-linked arabinofuranose side chains Cellvibrio japonicus L-arabinose + ?
-
 ir
L-arabinoxylan + H2O
-
 Streptomyces chartreusis GS901 L-arabinose + ?
-
 ir
methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
 Cellvibrio japonicus methylumbelliferone
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Cellvibrio japonicus p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis GS901 p-nitrophenol + L-arabinose
-
 ?
phenyl alpha-L-arabinofuranoside + H2O
-
 Pseudomonas fluorescens L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Bifidobacterium adolescentis L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Cellvibrio japonicus L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Thermoclostridium stercorarium L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces chartreusis GS901 L-arabinose + phenol
-
 ir
sugar beet arabinan + H2O
-
 Cellvibrio japonicus L-arabinofuranose + ?
-
 ?
wheat arabinoxylan + H2O
-
 Cellvibrio japonicus L-arabinofuranose + ?
-
 ?
wheat L-arabino-D-xylan + H2O
-
 Cellvibrio japonicus L-arabinose + ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Cellvibrio japonicus