Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arabitol | best inducer | Talaromyces purpureogenus | |
arabitol | best inducer | Aspergillus terreus |
Application | Comment | Organism |
---|---|---|
paper production | - |
Aspergillus niger |
paper production | - |
Aspergillus terreus |
paper production | enzyme may take part in the cellulose pulp biobleaching process | Talaromyces purpureogenus |
Cloned (Comment) | Organism |
---|---|
ABF2 gene cloned and expressed in Saccharomyces cerevisiae | Aspergillus niger |
abfB gene, nucleotide sequence determined | Aspergillus nidulans |
alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | strain B | Aspergillus niger | |
1.2 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Trichoderma reesei | |
1.23 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Talaromyces purpureogenus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus nidulans | - |
- |
extracellular | - |
Aspergillus niger | - |
- |
extracellular | - |
Talaromyces purpureogenus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
gel filtration | Talaromyces purpureogenus |
53000 | - |
- |
Aspergillus niger |
53000 | - |
- |
Trichoderma reesei |
58000 | - |
SDS-PAGE | Talaromyces purpureogenus |
60000 | - |
- |
Aspergillus niger |
62000 | - |
- |
Aspergillus awamori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beet arabinan + H2O | Aspergillus nidulans | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Talaromyces purpureogenus | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Aspergillus terreus | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Aspergillus niger B | - |
L-arabinose + ? | - |
ir | |
birchwood xylan + H2O | Aspergillus nidulans | - |
L-arabinose + ? | - |
? | |
birchwood xylan + H2O | Talaromyces purpureogenus | - |
L-arabinose + ? | - |
? | |
larchwood arabinogalactan + H2O | Talaromyces purpureogenus | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Talaromyces purpureogenus | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Aspergillus terreus | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Trichoderma reesei | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Aspergillus awamori | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Aspergillus awamori IFO 4033 | - |
L-arabinose + ? | - |
? | |
wheat bran + H2O | Talaromyces purpureogenus | - |
L-arabinose + ? | - |
? | |
wheat bran + H2O | Aspergillus terreus | - |
L-arabinose + ? | - |
? | |
wheat straw xylan + H2O | Talaromyces purpureogenus | - |
L-arabinose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
IFO4033 | - |
Aspergillus awamori IFO 4033 | - |
IFO4033 | - |
Aspergillus nidulans | - |
- |
- |
Aspergillus niger | - |
- |
- |
Aspergillus niger B | - |
- |
- |
Aspergillus terreus | - |
- |
- |
Aureobasidium pullulans | - |
- |
- |
Penicillium capsulatum | - |
- |
- |
Talaromyces purpureogenus | - |
- |
- |
Trichoderma reesei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Talaromyces purpureogenus |
- |
Aspergillus terreus |
- |
Aspergillus awamori |
- |
Aureobasidium pullulans |
2 isoenzymes | Penicillium capsulatum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58.4 | - |
- |
Talaromyces purpureogenus |
Storage Stability | Organism |
---|---|
4°C, at pH 2.5-7.5 retains more than 60% activity after 3 days, at pH 9 loses 90% of its activity in 20 h | Talaromyces purpureogenus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aryl-alpha-arabinofuranoside + H2O | - |
Talaromyces purpureogenus | L-arabinose + aryl alcohol | - |
? | |
beet arabinan + H2O | - |
Aspergillus nidulans | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Talaromyces purpureogenus | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Aspergillus terreus | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Aspergillus niger B | L-arabinose + ? | - |
ir | |
birchwood xylan + H2O | - |
Aspergillus nidulans | L-arabinose + ? | - |
? | |
birchwood xylan + H2O | - |
Talaromyces purpureogenus | L-arabinose + ? | - |
? | |
L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Talaromyces purpureogenus | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Trichoderma reesei | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Aspergillus niger B | L-arabinose + ? | - |
ir | |
larchwood arabinogalactan + H2O | - |
Talaromyces purpureogenus | L-arabinose + ? | - |
? | |
methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Talaromyces purpureogenus | methylumbelliferone | - |
? | |
additional information | - |
Aspergillus niger | ? | - |
? | |
additional information | - |
Aspergillus terreus | ? | - |
? | |
additional information | less than 2% activity with p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl alpha-L-arabinopyranoside, o-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-fucopyranoside, p-nitrophenyl-alpha-D-galactopyranoside | Talaromyces purpureogenus | ? | - |
? | |
additional information | - |
Aspergillus niger B | ? | - |
? | |
oat spelt xylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Talaromyces purpureogenus | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Aspergillus terreus | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Trichoderma reesei | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Aspergillus awamori | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Aspergillus awamori IFO 4033 | L-arabinose + ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Talaromyces purpureogenus | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger B | p-nitrophenol + L-arabinose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus nidulans | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Talaromyces purpureogenus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus terreus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium capsulatum | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus awamori | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aureobasidium pullulans | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus awamori IFO 4033 | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger B | L-arabinose + phenol | - |
ir | |
wheat bran + H2O | - |
Talaromyces purpureogenus | L-arabinose + ? | - |
? | |
wheat bran + H2O | - |
Aspergillus terreus | L-arabinose + ? | - |
? | |
wheat straw xylan + H2O | - |
Talaromyces purpureogenus | L-arabinose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Aspergillus niger |
monomer | - |
Trichoderma reesei |
monomer | 1 * 58000, SDS-PAGE | Talaromyces purpureogenus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus niger |
50 | - |
- |
Talaromyces purpureogenus |
50 | - |
- |
Aspergillus terreus |
60 | - |
- |
Aspergillus niger |
60 | - |
- |
Aspergillus terreus |
60 | - |
- |
Trichoderma reesei |
60 | - |
- |
Aspergillus awamori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | half-life of 2 h at 50°C and of 25 min at 55°C | Talaromyces purpureogenus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.7 | - |
- |
Aspergillus niger |
4 | - |
- |
Aspergillus niger |
4 | - |
- |
Talaromyces purpureogenus |
4 | - |
- |
Trichoderma reesei |