Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-arabinose | inducer of AF activity | Trichoderma reesei | |
sorbose | inducer of AF activity | Trichoderma reesei |
Cloned (Comment) | Organism |
---|---|
- |
Streptomyces lividans |
alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | isoenzyme AF53 | Trichoderma reesei | |
1.1 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | isoenzyme AF35 | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
AF35, SDS-PAGE | Trichoderma reesei |
53000 | - |
AF53, SDS-PAGE | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oat spelt xylan + H2O | Trichoderma reesei | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Trichoderma reesei PC-3-7 | - |
L-arabinose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | - |
66strain 1326 | - |
Streptomyces lividans 66 1326 | - |
66strain 1326 | - |
Trichoderma reesei | - |
PC-3-7 | - |
Trichoderma reesei PC-3-7 | - |
PC-3-7 | - |
Purification (Comment) | Organism |
---|---|
isoenzymes AF35 and AF53 | Trichoderma reesei |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
conidium | - |
Trichoderma reesei | - |
mycelium | - |
Trichoderma reesei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.061 | - |
isoenzyme AF35, oat spelt xylan as substrate | Trichoderma reesei |
2.6 | - |
isoenzyme AF53, oat spelt xylan as substrate | Trichoderma reesei |
120 | - |
isoenzyme AF53 | Trichoderma reesei |
196 | - |
isoenzyme AF35 | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinoxylan + H2O | - |
Trichoderma reesei | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Trichoderma reesei PC-3-7 | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + ? | - |
ir | |
oat spelt xylan + H2O | - |
Trichoderma reesei | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Trichoderma reesei PC-3-7 | L-arabinose + ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei PC-3-7 | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | p-nitrophenol + L-arabinose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei PC-3-7 | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + phenol | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Trichoderma reesei |
60 | - |
- |
Streptomyces lividans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Trichoderma reesei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | isoenzyme AF35 | Trichoderma reesei |
4.5 | 7 | isoenzyme AF53 | Trichoderma reesei |