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Literature summary for 3.2.1.55 extracted from

  • Schyns, P.J.Y.M.J.; de Frankrijker, J.; Zehnder, A.J.B.; Stams, A.J.M.
    Production, purification and characterization of an alpha-L-arabinofuranosidase from Bacteroides xylanolyticus X5-1 (1994), Appl. Microbiol. Biotechnol., 42, 548-554.
No PubMed abstract available
Search for more literature for 3.2.1.55

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Hungatella xylanolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Butyrivibrio fibrisolvens 5737
-
extracellular
-
 Aspergillus niger
-
-
extracellular
-
 Ruminococcus albus
-
-
extracellular
-
 Hungatella xylanolytica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 6 * 61000, SDS-PAGE Hungatella xylanolytica
364000
-
 gel filtration Hungatella xylanolytica

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Bacillus subtilis
-
-
-
Butyrivibrio fibrisolvens
-
 NCFB 2249
-
Butyrivibrio fibrisolvens
-
 GS113
-
Butyrivibrio fibrisolvens GS113
-
 GS113
-
Butyrivibrio fibrisolvens NCFB 2249
-
 NCFB 2249
-
Clostridium acetobutylicum
-
 ATCC 824
-
Hungatella xylanolytica
-
 X5-1, DSM 3808
-
Ruminococcus albus
-
 8
-
Ruminococcus albus 8
-
 8
-
Streptomyces purpurascens
-
 IFO 3389
-
Streptomyces sp.
-
 no. 17-1
-
Streptomyces sp. 17-1
-
 no. 17-1
-

Oxidation Stability

Oxidation Stability Organism
O2-stable Hungatella xylanolytica

Purification (Commentary)

Purification (Comment) Organism
-
 Ruminococcus albus
-
 Butyrivibrio fibrisolvens
-
 Hungatella xylanolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
108
-
-
 Hungatella xylanolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Bacillus subtilis ?
-
 ?
additional information
-
 Aspergillus niger ?
-
 ?
additional information
-
 Butyrivibrio fibrisolvens ?
-
 ?
additional information
-
 Streptomyces sp. ?
-
 ?
additional information
-
 Clostridium acetobutylicum ?
-
 ?
additional information
-
 Streptomyces purpurascens ?
-
 ?
additional information no activity with oat spelt xylan or arabinogalactan, not able to hydrolyse p-nitrophenyl-alpha-L-arabinopyranoside, xylobiose and xylotriose are not hydrolysed Hungatella xylanolytica ?
-
 ?
additional information
-
 Butyrivibrio fibrisolvens GS113 ?
-
 ?
additional information
-
 Butyrivibrio fibrisolvens NCFB 2249 ?
-
 ?
additional information
-
 Streptomyces sp. 17-1 ?
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Ruminococcus albus p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Clostridium acetobutylicum p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces purpurascens p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Hungatella xylanolytica p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Ruminococcus albus 8 p-nitrophenol + L-arabinose
-
 ?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. 17-1 p-nitrophenol + L-arabinose
-
 ?
phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Ruminococcus albus L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Butyrivibrio fibrisolvens L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Clostridium acetobutylicum L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces purpurascens L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Hungatella xylanolytica L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Ruminococcus albus 8 L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Butyrivibrio fibrisolvens GS113 L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Butyrivibrio fibrisolvens NCFB 2249 L-arabinose + phenol
-
 ir
phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. 17-1 L-arabinose + phenol
-
 ir

Subunits

Subunits Comment Organism
hexamer 6 * 61000, SDS-PAGE Hungatella xylanolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus niger
50
-
-
 Butyrivibrio fibrisolvens
50
-
-
 Hungatella xylanolytica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Hungatella xylanolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Hungatella xylanolytica

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9
-
 Hungatella xylanolytica