Cloned (Comment) | Organism |
---|---|
XS gene cloned and expressed in Escherichia coli, the recombinant 60 kDa protein shows XS and AF activities | Butyrivibrio fibrisolvens |
Crystallization (Comment) | Organism |
---|---|
- |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Streptomyces purpurascens | |
0.66 | - |
4-nitrophenyl alpha-L-arabinofuranoside | - |
Butyrivibrio fibrisolvens | |
0.7 | - |
4-nitrophenyl alpha-L-arabinofuranoside | - |
Butyrivibrio fibrisolvens | |
0.8 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Monilinia fructigena | |
1.3 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Ruminococcus albus | |
3.6 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Streptomyces sp. | |
4 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Clostridium acetobutylicum | |
4.9 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Butyrivibrio fibrisolvens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
8 * 31000 | Butyrivibrio fibrisolvens |
35000 | - |
1 * 35000 | Monilinia fructigena |
40000 | - |
- |
Monilinia fructigena |
53000 | - |
- |
Aspergillus niger |
62000 | - |
8 * 62000 | Streptomyces purpurascens |
65000 | - |
- |
Bacillus subtilis |
75000 | - |
4 * 75000, SDS-PAGE | Ruminococcus albus |
92000 | - |
strain 17-1, gel filtration | Streptomyces sp. |
94000 | - |
- |
Clostridium acetobutylicum |
240000 | - |
- |
Butyrivibrio fibrisolvens |
310000 | - |
- |
Ruminococcus albus |
495000 | - |
- |
Streptomyces purpurascens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beet arabinan + H2O | Bacillus subtilis | - |
L-arabinose + ? | - |
? | |
beet arabinan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
beet arabinan + H2O | Ruminococcus albus | - |
L-arabinose + ? | - |
? | |
beet arabinan + H2O | Butyrivibrio fibrisolvens | - |
L-arabinose + ? | - |
? | |
beet arabinan + H2O | Streptomyces sp. | - |
L-arabinose + ? | - |
? | |
beet arabinan + H2O | Clostridium acetobutylicum | - |
L-arabinose + ? | - |
? | |
beet arabinan + H2O | Streptomyces purpurascens | - |
L-arabinose + ? | - |
? | |
corn endosperm xylan + H2O | Butyrivibrio fibrisolvens | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Pseudomonas sp. | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Bacillus sp. (in: Bacteria) | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Butyrivibrio fibrisolvens | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Fibrobacter succinogenes | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Clostridium acetobutylicum | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Bacteroides ovatus | - |
L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | Prevotella ruminicola | - |
L-arabinose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Bacteroides ovatus | - |
- |
- |
Butyrivibrio fibrisolvens | - |
GS113 | - |
Butyrivibrio fibrisolvens GS113 | - |
GS113 | - |
Clostridium acetobutylicum | - |
ATCC 824 | - |
Fibrobacter succinogenes | - |
synonym Fibrobacter succinogenes | - |
Monilinia fructigena | - |
- |
- |
Prevotella ruminicola | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Ruminococcus albus | - |
- |
- |
Ruminococcus flavefaciens | - |
- |
- |
Streptomyces purpurascens | - |
IFO 3389 | - |
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
- |
Ruminococcus albus |
- |
Butyrivibrio fibrisolvens |
- |
Clostridium acetobutylicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Butyrivibrio fibrisolvens |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme loses less than 20% of its activity in 30 days | Butyrivibrio fibrisolvens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens GS113 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
arabinoxylobiose + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + xylobiose | - |
? | |
arabinoxylobiose + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + xylobiose | - |
? | |
beet arabinan + H2O | - |
Bacillus subtilis | L-arabinose + ? | - |
? | |
beet arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
beet arabinan + H2O | - |
Ruminococcus albus | L-arabinose + ? | - |
? | |
beet arabinan + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + ? | - |
? | |
beet arabinan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
? | |
beet arabinan + H2O | - |
Clostridium acetobutylicum | L-arabinose + ? | - |
? | |
beet arabinan + H2O | - |
Streptomyces purpurascens | L-arabinose + ? | - |
? | |
corn endosperm xylan + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + ? | - |
? | |
methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | methylumbelliferone | - |
? | |
methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens GS113 | methylumbelliferone | - |
? | |
additional information | - |
Bacillus subtilis | ? | - |
? | |
additional information | - |
Aspergillus niger | ? | - |
? | |
additional information | - |
Streptomyces sp. | ? | - |
? | |
additional information | - |
Clostridium acetobutylicum | ? | - |
? | |
additional information | - |
Streptomyces purpurascens | ? | - |
? | |
additional information | no release of sugars from cellulose, carboxymethyl cellulose or ara-arabinogalactan, no activity on arabinoxylobiose linked to coumaric or ferulic acid | Butyrivibrio fibrisolvens | ? | - |
? | |
additional information | no release of sugars from cellulose, carboxymethyl cellulose or ara-arabinogalactan, no activity on arabinoxylobiose linked to coumaric or ferulic acid | Butyrivibrio fibrisolvens GS113 | ? | - |
? | |
oat spelt xylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Pseudomonas sp. | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Bacillus sp. (in: Bacteria) | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Fibrobacter succinogenes | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Clostridium acetobutylicum | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Bacteroides ovatus | L-arabinose + ? | - |
? | |
oat spelt xylan + H2O | - |
Prevotella ruminicola | L-arabinose + ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Monilinia fructigena | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | p-nitrophenol + L-arabinose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Pseudomonas sp. | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus sp. (in: Bacteria) | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Fibrobacter succinogenes | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Bacteroides ovatus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Prevotella ruminicola | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus flavefaciens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Monilinia fructigena | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens GS113 | L-arabinose + phenol | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000 | Bacillus subtilis |
monomer | 1 * 35000 | Monilinia fructigena |
monomer | 1 * 53000 | Aspergillus niger |
monomer | 1 * 92000 | Streptomyces sp. |
monomer | 1 * 94000 | Clostridium acetobutylicum |
octamer | 8 * 62000 | Streptomyces purpurascens |
octamer | 8 * 31000 | Butyrivibrio fibrisolvens |
tetramer | 4 * 75000, SDS-PAGE | Ruminococcus albus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Butyrivibrio fibrisolvens |
55 | - |
- |
Aspergillus niger |
55 | - |
- |
Butyrivibrio fibrisolvens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
relatively stable up to | Butyrivibrio fibrisolvens |
55 | - |
stable to | Butyrivibrio fibrisolvens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | 4 | - |
Aspergillus niger |
4 | - |
- |
Aspergillus niger |
4 | - |
- |
Monilinia fructigena |
5 | 5.5 | - |
Clostridium acetobutylicum |
6 | 6.5 | - |
Aspergillus niger |
6 | 6.5 | - |
Butyrivibrio fibrisolvens |
6 | - |
- |
Butyrivibrio fibrisolvens |
6 | - |
- |
Streptomyces sp. |
6.5 | - |
- |
Bacillus subtilis |
6.5 | - |
- |
Streptomyces purpurascens |
6.9 | - |
- |
Ruminococcus albus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Streptomyces sp. |
4 | 8 | fairly stable | Butyrivibrio fibrisolvens |
5.5 | 8 | - |
Clostridium acetobutylicum |