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Literature summary for 3.2.1.55 extracted from

  • Hespell, R.B.; O'Bryan, P.
    Purification and characterization of an alpha-L-arabinofuranosidase from Butyrivibrio fibrisolvens GS113 (1992), Appl. Environ. Microbiol., 58, 1082-1088.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
XS gene cloned and expressed in Escherichia coli, the recombinant 60 kDa protein shows XS and AF activities Butyrivibrio fibrisolvens

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Streptomyces purpurascens
0.66
-
4-nitrophenyl alpha-L-arabinofuranoside
-
Butyrivibrio fibrisolvens
0.7
-
4-nitrophenyl alpha-L-arabinofuranoside
-
Butyrivibrio fibrisolvens
0.8
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Monilinia fructigena
1.3
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Ruminococcus albus
3.6
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Streptomyces sp.
4
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Clostridium acetobutylicum
4.9
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Butyrivibrio fibrisolvens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
8 * 31000 Butyrivibrio fibrisolvens
35000
-
1 * 35000 Monilinia fructigena
40000
-
-
Monilinia fructigena
53000
-
-
Aspergillus niger
62000
-
8 * 62000 Streptomyces purpurascens
65000
-
-
Bacillus subtilis
75000
-
4 * 75000, SDS-PAGE Ruminococcus albus
92000
-
strain 17-1, gel filtration Streptomyces sp.
94000
-
-
Clostridium acetobutylicum
240000
-
-
Butyrivibrio fibrisolvens
310000
-
-
Ruminococcus albus
495000
-
-
Streptomyces purpurascens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Bacillus subtilis
-
L-arabinose + ?
-
?
beet arabinan + H2O Aspergillus niger
-
L-arabinose + ?
-
?
beet arabinan + H2O Ruminococcus albus
-
L-arabinose + ?
-
?
beet arabinan + H2O Butyrivibrio fibrisolvens
-
L-arabinose + ?
-
?
beet arabinan + H2O Streptomyces sp.
-
L-arabinose + ?
-
?
beet arabinan + H2O Clostridium acetobutylicum
-
L-arabinose + ?
-
?
beet arabinan + H2O Streptomyces purpurascens
-
L-arabinose + ?
-
?
corn endosperm xylan + H2O Butyrivibrio fibrisolvens
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Aspergillus niger
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Pseudomonas sp.
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Bacillus sp. (in: Bacteria)
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Butyrivibrio fibrisolvens
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Fibrobacter succinogenes
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Clostridium acetobutylicum
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Bacteroides ovatus
-
L-arabinose + ?
-
?
oat spelt xylan + H2O Prevotella ruminicola
-
L-arabinose + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus subtilis
-
-
-
Bacteroides ovatus
-
-
-
Butyrivibrio fibrisolvens
-
GS113
-
Butyrivibrio fibrisolvens GS113
-
GS113
-
Clostridium acetobutylicum
-
ATCC 824
-
Fibrobacter succinogenes
-
synonym Fibrobacter succinogenes
-
Monilinia fructigena
-
-
-
Prevotella ruminicola
-
-
-
Pseudomonas sp.
-
-
-
Ruminococcus albus
-
-
-
Ruminococcus flavefaciens
-
-
-
Streptomyces purpurascens
-
IFO 3389
-
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus niger
-
Ruminococcus albus
-
Butyrivibrio fibrisolvens
-
Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.1
-
-
Butyrivibrio fibrisolvens

Storage Stability

Storage Stability Organism
-20°C, purified enzyme loses less than 20% of its activity in 30 days Butyrivibrio fibrisolvens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens GS113 4-nitrophenol + alpha-L-arabinofuranose
-
?
arabinoxylobiose + H2O
-
Butyrivibrio fibrisolvens L-arabinose + xylobiose
-
?
arabinoxylobiose + H2O
-
Butyrivibrio fibrisolvens GS113 L-arabinose + xylobiose
-
?
beet arabinan + H2O
-
Bacillus subtilis L-arabinose + ?
-
?
beet arabinan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
beet arabinan + H2O
-
Ruminococcus albus L-arabinose + ?
-
?
beet arabinan + H2O
-
Butyrivibrio fibrisolvens L-arabinose + ?
-
?
beet arabinan + H2O
-
Streptomyces sp. L-arabinose + ?
-
?
beet arabinan + H2O
-
Clostridium acetobutylicum L-arabinose + ?
-
?
beet arabinan + H2O
-
Streptomyces purpurascens L-arabinose + ?
-
?
corn endosperm xylan + H2O
-
Butyrivibrio fibrisolvens L-arabinose + ?
-
?
methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens methylumbelliferone
-
?
methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens GS113 methylumbelliferone
-
?
additional information
-
Bacillus subtilis ?
-
?
additional information
-
Aspergillus niger ?
-
?
additional information
-
Streptomyces sp. ?
-
?
additional information
-
Clostridium acetobutylicum ?
-
?
additional information
-
Streptomyces purpurascens ?
-
?
additional information no release of sugars from cellulose, carboxymethyl cellulose or ara-arabinogalactan, no activity on arabinoxylobiose linked to coumaric or ferulic acid Butyrivibrio fibrisolvens ?
-
?
additional information no release of sugars from cellulose, carboxymethyl cellulose or ara-arabinogalactan, no activity on arabinoxylobiose linked to coumaric or ferulic acid Butyrivibrio fibrisolvens GS113 ?
-
?
oat spelt xylan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
oat spelt xylan + H2O
-
Pseudomonas sp. L-arabinose + ?
-
?
oat spelt xylan + H2O
-
Bacillus sp. (in: Bacteria) L-arabinose + ?
-
?
oat spelt xylan + H2O
-
Butyrivibrio fibrisolvens L-arabinose + ?
-
?
oat spelt xylan + H2O
-
Fibrobacter succinogenes L-arabinose + ?
-
?
oat spelt xylan + H2O
-
Clostridium acetobutylicum L-arabinose + ?
-
?
oat spelt xylan + H2O
-
Bacteroides ovatus L-arabinose + ?
-
?
oat spelt xylan + H2O
-
Prevotella ruminicola L-arabinose + ?
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aspergillus niger p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Ruminococcus albus p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces sp. p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Clostridium acetobutylicum p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Monilinia fructigena p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces purpurascens p-nitrophenol + L-arabinose
-
?
phenyl alpha-L-arabinofuranoside + H2O
-
Bacillus subtilis L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Aspergillus niger L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Pseudomonas sp. L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Bacillus sp. (in: Bacteria) L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Ruminococcus albus L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces sp. L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Fibrobacter succinogenes L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Clostridium acetobutylicum L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Bacteroides ovatus L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Prevotella ruminicola L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Ruminococcus flavefaciens L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Monilinia fructigena L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Streptomyces purpurascens L-arabinose + phenol
-
ir
phenyl alpha-L-arabinofuranoside + H2O
-
Butyrivibrio fibrisolvens GS113 L-arabinose + phenol
-
ir

Subunits

Subunits Comment Organism
monomer 1 * 65000 Bacillus subtilis
monomer 1 * 35000 Monilinia fructigena
monomer 1 * 53000 Aspergillus niger
monomer 1 * 92000 Streptomyces sp.
monomer 1 * 94000 Clostridium acetobutylicum
octamer 8 * 62000 Streptomyces purpurascens
octamer 8 * 31000 Butyrivibrio fibrisolvens
tetramer 4 * 75000, SDS-PAGE Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Butyrivibrio fibrisolvens
55
-
-
Aspergillus niger
55
-
-
Butyrivibrio fibrisolvens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
relatively stable up to Butyrivibrio fibrisolvens
55
-
stable to Butyrivibrio fibrisolvens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8 4
-
Aspergillus niger
4
-
-
Aspergillus niger
4
-
-
Monilinia fructigena
5 5.5
-
Clostridium acetobutylicum
6 6.5
-
Aspergillus niger
6 6.5
-
Butyrivibrio fibrisolvens
6
-
-
Butyrivibrio fibrisolvens
6
-
-
Streptomyces sp.
6.5
-
-
Bacillus subtilis
6.5
-
-
Streptomyces purpurascens
6.9
-
-
Ruminococcus albus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9
-
Streptomyces sp.
4 8 fairly stable Butyrivibrio fibrisolvens
5.5 8
-
Clostridium acetobutylicum