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Literature summary for 3.2.1.55 extracted from

  • Kaji, A.; Sato, M.; Tsutsui, Y.
    An alpha-L-arabinofuranosidase produced by wild-type Streptomyces sp. No. 17-1 (1981), Agric. Biol. Chem., 45, 925-931.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Aspergillus niger
Ag+
-
Rhodotorula flava
Ag+
-
Streptomyces purpurascens
Ag+
-
Streptomyces sp.
Ca2+
-
Streptomyces sp.
Hg2+
-
Pectobacterium carotovorum
Hg2+
-
Rhodotorula flava
Hg2+
-
Streptomyces purpurascens
Hg2+ 1 mM Hg(Ch3COO)2 Streptomyces sp.
p-chloromercuribenzoate
-
Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
strain 17-1, gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beet arabinan + H2O Aspergillus niger
-
L-arabinose + ?
-
?
beet arabinan + H2O Pectobacterium carotovorum
-
L-arabinose + ?
-
?
beet arabinan + H2O Streptomyces sp.
-
L-arabinose + ?
-
?
beet arabinan + H2O Athelia rolfsii
-
L-arabinose + ?
-
?
beet arabinan + H2O Rhodotorula flava
-
L-arabinose + ?
-
?
beet arabinan + H2O Streptomyces purpurascens
-
L-arabinose + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Athelia rolfsii
-
-
-
Clostridium felsineum
-
-
-
Kitasatospora aureofaciens
-
-
-
Pectobacterium carotovorum
-
-
-
Rhodotorula flava
-
-
-
Streptomyces flaveolus
-
-
-
Streptomyces lavendulae
-
-
-
Streptomyces massasporeus
-
-
-
Streptomyces olivochromogenes
-
-
-
Streptomyces purpurascens
-
IFO 3389
-
Streptomyces sp.
-
no. 003
-
Streptomyces sp.
-
no. 17-1
-
Streptomyces sp. 17-1
-
no. 17-1
-
Streptomyces sp. 3
-
no. 003
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
-
Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-arabinan + H2O
-
Streptomyces sp. L-arabinose
-
?
1,3-arabinan + H2O
-
Streptomyces sp. 17-1 L-arabinose
-
?
1,3-arabinan + H2O
-
Streptomyces sp. 3 L-arabinose
-
?
1,5-L-arabinan + H2O
-
Aspergillus niger L-arabinose + ?
-
ir
1,5-L-arabinan + H2O
-
Streptomyces sp. L-arabinose + ?
-
ir
1,5-L-arabinan + H2O
-
Athelia rolfsii L-arabinose + ?
-
ir
1,5-L-arabinan + H2O
-
Rhodotorula flava L-arabinose + ?
-
ir
1,5-L-arabinan + H2O
-
Streptomyces sp. 17-1 L-arabinose + ?
-
ir
1,5-L-arabinan + H2O
-
Streptomyces sp. 3 L-arabinose + ?
-
ir
beet arabinan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
beet arabinan + H2O
-
Pectobacterium carotovorum L-arabinose + ?
-
?
beet arabinan + H2O
-
Streptomyces sp. L-arabinose + ?
-
?
beet arabinan + H2O
-
Athelia rolfsii L-arabinose + ?
-
?
beet arabinan + H2O
-
Rhodotorula flava L-arabinose + ?
-
?
beet arabinan + H2O
-
Streptomyces purpurascens L-arabinose + ?
-
?
L-arabinan + H2O
-
Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces olivochromogenes (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Kitasatospora aureofaciens (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces lavendulae (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces purpurascens (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinan + H2O
-
Streptomyces flaveolus (1,5-alpha-L-arabinose)25 + ?
-
?
L-arabinogalactan + H2O
-
Aspergillus niger L-arabinose + ?
-
?
L-arabinogalactan + H2O
-
Streptomyces sp. L-arabinose + ?
-
?
L-arabinogalactan + H2O
-
Streptomyces sp. 17-1 L-arabinose + ?
-
?
L-arabinogalactan + H2O
-
Streptomyces sp. 3 L-arabinose + ?
-
?
L-arabinoxylan + H2O
-
Aspergillus niger L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Streptomyces sp. L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Rhodotorula flava L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Streptomyces sp. 17-1 L-arabinose + ?
-
ir
L-arabinoxylan + H2O
-
Streptomyces sp. 3 L-arabinose + ?
-
ir
additional information
-
Aspergillus niger ?
-
?
additional information
-
Pectobacterium carotovorum ?
-
?
additional information
-
Rhodotorula flava ?
-
?
additional information
-
Streptomyces purpurascens ?
-
?
additional information inactive on gum arabic and p-nitrophenyl-alpha- and beta-galactopyranosides, purified enzyme is inactive on xylan, galactan, soluble starch, carboxymethyl cellulose, xyloside, glycoside, mannoside, fucoside, N-acetyl-glucosaminides and N-acetyl-galactosaminides Streptomyces sp. ?
-
?
additional information inactive on gum arabic and p-nitrophenyl-alpha- and beta-galactopyranosides, purified enzyme is inactive on xylan, galactan, soluble starch, carboxymethyl cellulose, xyloside, glycoside, mannoside, fucoside, N-acetyl-glucosaminides and N-acetyl-galactosaminides Streptomyces sp. 17-1 ?
-
?
additional information inactive on gum arabic and p-nitrophenyl-alpha- and beta-galactopyranosides, purified enzyme is inactive on xylan, galactan, soluble starch, carboxymethyl cellulose, xyloside, glycoside, mannoside, fucoside, N-acetyl-glucosaminides and N-acetyl-galactosaminides Streptomyces sp. 3 ?
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aspergillus niger p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces sp. p-nitrophenol + L-arabinose
-
?
p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Streptomyces purpurascens p-nitrophenol + L-arabinose
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pectobacterium carotovorum
6
-
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 stable for 72 h at 2°C, loses 38% of its original activity at pH 9.0 Streptomyces sp.