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Literature summary for 3.2.1.54 extracted from
- Genetic and biochemical characterization of a novel thermostable cyclomaltodextrinase from Anoxybacillus flavithermus (2019), Starch, 71, 180033.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 5 mM, 24% increase in enzyme activity | Anoxybacillus flavithermus subsp. flavithermus |  |
| dithiothreitol | 5 mM, 95% increase in enzyme activity | Anoxybacillus flavithermus subsp. flavithermus | |
| EDTA | 5 mM, 34% increase in enzyme activity | Anoxybacillus flavithermus subsp. flavithermus | |
| K+ | 5 mM, enzyme activity increases by about 49% | Anoxybacillus flavithermus subsp. flavithermus | |
| Na+ | 5 mM, enzyme activity increases by about 34% | Anoxybacillus flavithermus subsp. flavithermus | |
| PMSF | 5 mM, 28% increase in enzyme activity | Anoxybacillus flavithermus subsp. flavithermus | |
| Zn2+ | 5 mM, enzyme activity increases by about 16% | Anoxybacillus flavithermus subsp. flavithermus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Anoxybacillus flavithermus subsp. flavithermus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, activity decreases by about 66% | Anoxybacillus flavithermus subsp. flavithermus | |
| Co2+ | 5 mM, activity decreases by about 91% | Anoxybacillus flavithermus subsp. flavithermus | |
| Cu2+ | - |
Anoxybacillus flavithermus subsp. flavithermus | |
| Fe2+ | - |
Anoxybacillus flavithermus subsp. flavithermus | |
| Mg2+ | 5 mM, activity decreases by about 31% | Anoxybacillus flavithermus subsp. flavithermus | |
| PMSF | 10 mM, activity decreases by about 50% | Anoxybacillus flavithermus subsp. flavithermus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
alpha-cyclodextrin | Km-value: 3.99 mg/ml, pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
| additional information | - |
beta-cyclodextrin | Km-value: 3.45 mg/ml, pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
| additional information | - |
gamma-cyclodextrin | Km-value: 2.98 mg/ml, pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Anoxybacillus flavithermus subsp. flavithermus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68800 | - |
calculated | Anoxybacillus flavithermus subsp. flavithermus |
| 72000 | - |
SDS-PAGE | Anoxybacillus flavithermus subsp. flavithermus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anoxybacillus flavithermus subsp. flavithermus | A0A0Y0H9K3 | - |
- |
| Anoxybacillus flavithermus subsp. flavithermus ZNU.NGA | A0A0Y0H9K3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Anoxybacillus flavithermus subsp. flavithermus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | glucose and maltose are the main products | Anoxybacillus flavithermus subsp. flavithermus | maltose + glucose | - |
? | |
| alpha-cyclodextrin + H2O | glucose and maltose are the main products | Anoxybacillus flavithermus subsp. flavithermus ZNU.NGA | maltose + glucose | - |
? | |
| beta-cyclodextrin + H2O | glucose and maltose are the main products. About 75% of the activity as compared to alpha-cyclodextrin | Anoxybacillus flavithermus subsp. flavithermus | maltose + glucose | - |
? | |
| beta-cyclodextrin + H2O | glucose and maltose are the main products. About 75% of the activity as compared to alpha-cyclodextrin | Anoxybacillus flavithermus subsp. flavithermus ZNU.NGA | maltose + glucose | - |
? | |
| gamma-cyclodextrin + H2O | glucose and maltose are the main products. About 60% of the activity as compared to alpha-cyclodextrin | Anoxybacillus flavithermus subsp. flavithermus | maltose + glucose | - |
? | |
| gamma-cyclodextrin + H2O | glucose and maltose are the main products. About 60% of the activity as compared to alpha-cyclodextrin | Anoxybacillus flavithermus subsp. flavithermus ZNU.NGA | maltose + glucose | - |
? | |
| glycogen + H2O | less than 5% of the activity as compared to alpha-cyclodextrin | Anoxybacillus flavithermus subsp. flavithermus | ? | - |
? | |
| glycogen + H2O | less than 5% of the activity as compared to alpha-cyclodextrin | Anoxybacillus flavithermus subsp. flavithermus ZNU.NGA | ? | - |
? | |
| starch + H2O | less than 5% of the activity as compared to alpha-cyclodextrin | Anoxybacillus flavithermus subsp. flavithermus | ? | - |
? | |
| starch + H2O | less than 5% of the activity as compared to alpha-cyclodextrin | Anoxybacillus flavithermus subsp. flavithermus ZNU.NGA | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | - |
Anoxybacillus flavithermus subsp. flavithermus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDase | - |
Anoxybacillus flavithermus subsp. flavithermus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Anoxybacillus flavithermus subsp. flavithermus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 75 | 40°C: about 40% of maximal activity, 75°C: about 45% of maximal activity | Anoxybacillus flavithermus subsp. flavithermus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
60 min, the enzyme retains 70% of its initial activity | Anoxybacillus flavithermus subsp. flavithermus |
| 65 | - |
100 min, the enzyme retains 50% of its initial activity | Anoxybacillus flavithermus subsp. flavithermus |
| 70 | - |
30 min, the enzyme retains 50% of its initial activity | Anoxybacillus flavithermus subsp. flavithermus |
| 75 | - |
5 min, complete loss of activity | Anoxybacillus flavithermus subsp. flavithermus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 308 | - |
gamma-cyclodextrin | pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
| 382 | - |
beta-cyclodextrin | pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
| 580 | - |
alpha-cyclodextrin | pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Anoxybacillus flavithermus subsp. flavithermus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.0: about 30% of maximal activity, pH 8.5: about 30% of maximal activity | Anoxybacillus flavithermus subsp. flavithermus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
alpha-cyclodextrin | kcat/Km-value: 145.36 mg/ml*s, pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
| additional information | - |
beta-cyclodextrin | kcat/Km-value: 110.72 mg/ml*s, pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
| additional information | - |
gamma-cyclodextrin | kcat/Km-value: 103.36 mg/ml*s, pH 7.0, 65°C | Anoxybacillus flavithermus subsp. flavithermus | |
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