Literature summary for 3.2.1.54 extracted from

Lunina, N.A.; Agafonova, E.V.; Chekanovskaya, L.A.; Dvortsov, I.A.; Berezina, O.V.; Shedova, E.N.; Kostrov, S.V.; Velikodvorskaya, G.A.
Co-expression of the Thermotoga neapolitana aglB gene with an upstream 3-coding fragment of the malG gene improves enzymatic characteristics of recombinant AglB cyclomaltodextrinase (2007), Protein Expr. Purif., 54, 18-23.

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Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	at 5 mM for isoenzyme ag1B* and ag1B(1Met), no activity against ag1B(2Met)	Thermotoga neapolitana

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli, three expression vectors are constructed, aglB gene linked to an upstream 90-bp 30-terminal region of the malG gene with the stop codon overlapping with the start codon of aglB, aglB with two tandem potential start codons and ag1B with only one start codon	Thermotoga neapolitana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.23	-	p-nitrophenyl-D-maltopentaoside	isoenzyme ag1B*	Thermotoga neapolitana
0.27	-	p-nitrophenyl-D-maltopentaoside	isoenzyme ag1B(1Met)	Thermotoga neapolitana
1.72	-	p-nitrophenyl-D-maltopentaoside	isoenzyme ag1B(2Met)	Thermotoga neapolitana
3.6	-	beta-cyclodextrin	isoenzyme ag1B(1Met)	Thermotoga neapolitana
3.9	-	beta-cyclodextrin	isoenzyme ag1B*	Thermotoga neapolitana
6.06	-	beta-cyclodextrin	isoenzyme ag1B(2Met)	Thermotoga neapolitana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	SDS-PAGE, aglB*	Thermotoga neapolitana

Organism

Organism	UniProt	Comment	Textmining
Thermotoga neapolitana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by DEAE-Toyopearl chromatography and ultrafiltration	Thermotoga neapolitana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1	-	isoenzyme aglB(2Met) after purification, beta-cyclodextrin as substrate	Thermotoga neapolitana
22.4	-	isoenzyme aglB(1Met) after purification, beta-cyclodextrin as substrate	Thermotoga neapolitana
22.8	-	isoenzyme aglB* after purification, beta-cyclodextrin as substrate	Thermotoga neapolitana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-cyclodextrin + H2O	-	Thermotoga neapolitana	maltooligosaccharides	maltohexaose, maltoheptaose, maltooctaose	?
beta-cyclodextrin + H2O	-	Thermotoga neapolitana	maltooligosaccharides	maltohexaose, maltoheptaose, maltooctaose	?
cyclomaltodextrin + H2O	-	Thermotoga neapolitana	linear maltodextrin	-	?
gamma-cyclodextrin + H2O	preferred substrate	Thermotoga neapolitana	maltooligosaccharides	maltohexaose, maltoheptaose, maltooctaose	?
p-nitrophenyl-D-maltopentaoside + H2O	-	Thermotoga neapolitana	p-nitrophenol + maltopentaose	-	?

Subunits

Subunits	Comment	Organism
octamer	8 * 55000, aglB*	Thermotoga neapolitana
oligomer	aglB(2Met) higher aggregated	Thermotoga neapolitana
tetramer	4 * 55000, aglB*	Thermotoga neapolitana

Synonyms

Synonyms	Comment	Organism
AglB	-	Thermotoga neapolitana
CDase	-	Thermotoga neapolitana
cyclodextrinase	-	Thermotoga neapolitana
cyclomaltodextrinase	-	Thermotoga neapolitana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	-	Thermotoga neapolitana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	half-inactivation time of AglB(2Met) at 90°C is 5 min, for AglB* and AglB(1Met) 3 h	Thermotoga neapolitana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Thermotoga neapolitana

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Release 2023.1 (January 2023)