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Literature summary for 3.2.1.54 extracted from
- Purification and characterization of cyclodextrinase from Paenibacillus sp. A11 (2005), Enzyme Microb. Technol., 36, 168-175.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide | 5 mM, 37% inhibition | Paenibacillus sp. |  |
| acarbose | 5 mM, 91% inhibition | Paenibacillus sp. | |
| AgNO3 | 10 mM, complete inhibition | Paenibacillus sp. | |
| CuSO4 | 10 mM, complete inhibition | Paenibacillus sp. | |
| diethylpyrocarbonate | 1 mM, complete inhibition | Paenibacillus sp. | |
| FeSO4 | 10 mM, 68% inhibition | Paenibacillus sp. | |
| HgCl2 | 10 mM, complete inhibition | Paenibacillus sp. | |
| iodoacetamide | 5 mM, 49% inhibition | Paenibacillus sp. | |
| MgCl2 | 10 mM, 35% inhibition | Paenibacillus sp. | |
| MnCl2 | 10 mM, 33% inhibition | Paenibacillus sp. | |
| N-bromosuccinimide | 89% inhibition at 0.1 mM, complete inhibition at 1 mM | Paenibacillus sp. | |
| Phenylglyoxal | 5 mM, 60% inhibition | Paenibacillus sp. | |
| ZnCl2 | 10 mM, 78% inhibition | Paenibacillus sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.47 | - |
beta-cyclodextrin | pH 7.0, 40°C | Paenibacillus sp. | |
| 3.1 | - |
gamma-cyclodextrin | pH 7.0, 40°C | Paenibacillus sp. | |
| 5.66 | - |
alpha-cyclodextrin | pH 7.0, 40°C | Paenibacillus sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Paenibacillus sp. | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
gel filtration | Paenibacillus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Paenibacillus sp. | inducible enzyme | ? | - |
? | |
| additional information | Paenibacillus sp. A11 | inducible enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus sp. | - |
- |
- |
| Paenibacillus sp. A11 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paenibacillus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Paenibacillus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | 53% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose | main products | ? | |
| beta-cyclodextrin + H2O | - |
Paenibacillus sp. | D-glucose + maltose | main products | ? | |
| dextrin + H2O | 29% of the activity with beta-cyclodextrin | Paenibacillus sp. | ? | - |
? | |
| dextrin + H2O | 29% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | ? | - |
? | |
| G1-alpha-cyclodextrin + H2O | 24% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose | main products | ? | |
| G1-beta-cyclodextrin + H2O | 32% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose | main products | ? | |
| G2-alpha-cyclodextrin + H2O | 19% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose | main products | ? | |
| G2-beta-cyclodextrin + H2O | 30% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose | main products | ? | |
| gamma-cyclodextrin + H2O | 59% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose | main products | ? | |
| maltoheptaose + H2O | 41% of the activity with beta-cyclodextrin | Paenibacillus sp. | ? | - |
? | |
| maltohexaose + H2O | 33% of the activity with beta-cyclodextrin | Paenibacillus sp. | ? | - |
? | |
| maltohexaose + H2O | 33% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | ? | - |
? | |
| maltopentaose + H2O | 18% of the activity with beta-cyclodextrin | Paenibacillus sp. | ? | - |
? | |
| maltopentaose + H2O | 18% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | ? | - |
? | |
| maltotetraose + H2O | 12% of the activity with beta-cyclodextrin | Paenibacillus sp. | ? | - |
? | |
| maltotetraose + H2O | 12% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | ? | - |
? | |
| maltotriose + H2O | 11% of the activity with beta-cyclodextrin | Paenibacillus sp. | ? | - |
? | |
| additional information | inducible enzyme | Paenibacillus sp. | ? | - |
? | |
| additional information | inducible enzyme | Paenibacillus sp. A11 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 80000, SDS-PAGE | Paenibacillus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Paenibacillus sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | 20°C: about 65% of maximal activity, 70°C: about 40% of maximal activity | Paenibacillus sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 800 | - |
alpha-cyclodextrin | pH 7.0, 40°C | Paenibacillus sp. | |
| 1155 | - |
gamma-cyclodextrin | pH 7.0, 40°C | Paenibacillus sp. | |
| 2044 | - |
beta-cyclodextrin | pH 7.0, 40°C | Paenibacillus sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Paenibacillus sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | about 50% of maximal activity at pH 5.0 and 8.0 | Paenibacillus sp. |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Paenibacillus sp. | isoeletric focusing, pH-range 3-9 | - |
5.4 |
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