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Literature summary for 3.2.1.54 extracted from
- Purification and properties of the cyclodextrinase of Bacillus sphaericus ATCC 7055 (1994), Appl. Microbiol. Biotechnol., 42, 46-50.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-D-glucose | 0.01 M, relative activity 140% | Lysinibacillus sphaericus |  |
| glucosamine | 0.01 M, relative activity 133% | Lysinibacillus sphaericus | |
| glucose | 0.01 M, relative activity 143% | Lysinibacillus sphaericus | |
| glucose 6-phosphate | 0.005 M, relative activity 111% | Lysinibacillus sphaericus | |
| maltose | 0.01 M, relative activity 140% | Lysinibacillus sphaericus | |
| miglitol | 1 mg/ml, relative activity 107% | Lysinibacillus sphaericus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acarbose | - |
Lysinibacillus sphaericus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.9 | - |
beta-cyclodextrin | - |
Lysinibacillus sphaericus | |
| 4 | - |
alpha-cyclodextrin | - |
Lysinibacillus sphaericus | |
| 4.2 | - |
gamma-cyclodextrin | - |
Lysinibacillus sphaericus | |
| 5.8 | - |
maltoheptaose | - |
Lysinibacillus sphaericus | |
| 6.1 | - |
maltohexaose | - |
Lysinibacillus sphaericus | |
| 7.5 | - |
maltotetraose | - |
Lysinibacillus sphaericus | |
| 14 | - |
maltotriose | - |
Lysinibacillus sphaericus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 91200 | 95000 | gel filtration | Lysinibacillus sphaericus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lysinibacillus sphaericus | - |
ATCC 7055 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lysinibacillus sphaericus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 366.3 | - |
homogenous by SDS-PAGE | Lysinibacillus sphaericus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclomaltodextrin + H2O | - |
Lysinibacillus sphaericus | alpha-D-glucose + maltose | - |
? | |
| beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | maltose + glucose | - |
? | |
| beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | glucose + maltose + maltotriose + maltotetraose | - |
? | |
| branched cyclodextrins + H2O | - |
Lysinibacillus sphaericus | glucose + maltose | - |
? | |
| cyclomaltodextrin + H2O | - |
Lysinibacillus sphaericus | maltodextrin | - |
? | |
| gamma-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | glucose + maltose + maltotriose + maltotetraose + maltopentaose + maltohexaose + maltoheptaose + maltooctaose | - |
? | |
| glucosyl-beta-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | 6,4-O-alpha-D-glucosylmaltoheptaose | - |
? | |
| maltoheptaose + H2O | - |
Lysinibacillus sphaericus | D-glucose + maltose + ? | - |
? | |
| maltopentaose + H2O | - |
Lysinibacillus sphaericus | maltose + maltotriose | - |
? | |
| maltosyl-alpha-cyclodextrin + H2O | - |
Lysinibacillus sphaericus | 6,3-O-alpha-D-maltosylmaltohexaose | - |
? | |
| maltosyl-beta-cyclodextrin | - |
Lysinibacillus sphaericus | 6,4-O-alpha-D-maltosylmaltoheptaose | - |
? | |
| pullulan + H2O | - |
Lysinibacillus sphaericus | glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds | - |
? | |
| starch + H2O | - |
Lysinibacillus sphaericus | glucose + maltose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Lysinibacillus sphaericus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
100% stable for 30 min up to 40°C | Lysinibacillus sphaericus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | 97% activity at pH 7 | Lysinibacillus sphaericus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | 100% stable | Lysinibacillus sphaericus |
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