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Literature summary for 3.2.1.54 extracted from
- Structure of the gene encoding cyclomaltodextrinase from Clostridium thermohydrosulfuricum 39E and characterization of the enzyme purified from Escherichia coli (1992), J. Bacteriol., 8, 5400-5405.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid pPG22, expression in Escherichia coli | Thermoanaerobacter thermohydrosulfuricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Thermoanaerobacter thermohydrosulfuricus |  |
| Cu2+ | - |
Thermoanaerobacter thermohydrosulfuricus | |
| Hg2+ | - |
Thermoanaerobacter thermohydrosulfuricus | |
| N-bromosuccinimide | - |
Thermoanaerobacter thermohydrosulfuricus | |
| p-chloromercuribenzoate | - |
Thermoanaerobacter thermohydrosulfuricus | |
| Zn2+ | - |
Thermoanaerobacter thermohydrosulfuricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
gamma-cyclodextrin | - |
Thermoanaerobacter thermohydrosulfuricus | |
| 2.1 | - |
beta-cyclodextrin | - |
Thermoanaerobacter thermohydrosulfuricus | |
| 2.5 | - |
alpha-cyclodextrin | - |
Thermoanaerobacter thermohydrosulfuricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
monomer SDS-PAGE | Thermoanaerobacter thermohydrosulfuricus |
| 68030 | - |
denatured protein estimated by SDS-PAGE, native enzyme determined by gel filtration | Thermoanaerobacter thermohydrosulfuricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter thermohydrosulfuricus | - |
39E | - |
| Thermoanaerobacter thermohydrosulfuricus 39E | - |
39E | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gene encoding enzyme cloned in Escherichia coli, expressed and purified 12fold from Escherichia coli | Thermoanaerobacter thermohydrosulfuricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 62 | - |
gel filtration | Thermoanaerobacter thermohydrosulfuricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + D-glucose | - |
? | |
| amylopectin + H2O | rate of hydrolysis less than 5% of the rate of alpha-cyclodextrin hydrolysis | Thermoanaerobacter thermohydrosulfuricus | maltose + ? | - |
? | |
| amylose + H2O | less than 5% of the rate of alpha-cyclodextrin hydrolysis | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
| beta-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | glucose + maltose + long chain linear oligomers | - |
? | |
| beta-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | glucose + maltose + long chain linear oligomers | - |
? | |
| cyclomaltodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltodextrin | - |
? | |
| cyclomaltodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus 39E | maltodextrin | - |
? | |
| gamma-cyclodextrin + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + D-glucose | - |
? | |
| glycogen + H2O | relative rate of hydrolysis 0,1% | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
| maltodextrins + H2O | linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain | Thermoanaerobacter thermohydrosulfuricus | maltose + glucose | - |
? | |
| maltodextrins + H2O | linear, enzyme degrades the linear molecules by splitting off maltose units from the nonreducing end of the chain | Thermoanaerobacter thermohydrosulfuricus 39E | maltose + glucose | - |
? | |
| maltoheptaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | D-glucose + maltose + ? | - |
? | |
| maltohexaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltotetraose + maltose | - |
? | |
| maltopentaose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | maltose + maltotriose | - |
? | |
| maltotetraose + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | 2 maltose | - |
? | |
| maltotriose + H2O | slowly | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
| maltotriose + H2O | slowly | Thermoanaerobacter thermohydrosulfuricus 39E | ? | - |
? | |
| additional information | not: maltose | Thermoanaerobacter thermohydrosulfuricus | ? | - |
? | |
| additional information | not: maltose | Thermoanaerobacter thermohydrosulfuricus 39E | ? | - |
? | |
| pullulan + H2O | rate of hydrolysis less than 5% of the rate of alpha-cyclodextrin hydrolysis | Thermoanaerobacter thermohydrosulfuricus | glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds | - |
? | |
| starch + H2O | - |
Thermoanaerobacter thermohydrosulfuricus | glucose + maltose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| thermophilic CDase | - |
Thermoanaerobacter thermohydrosulfuricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
beta-cyclodextrin as substrate | Thermoanaerobacter thermohydrosulfuricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 75 | - |
Thermoanaerobacter thermohydrosulfuricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
incubation for 1 h led to a 15% loss of activity | Thermoanaerobacter thermohydrosulfuricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
- |
Thermoanaerobacter thermohydrosulfuricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | - |
Thermoanaerobacter thermohydrosulfuricus |
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