Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paenibacillus sp. |
Protein Variants | Comment | Organism |
---|---|---|
D607E | Km is increased by 5-fold, the kcat is decreased to 1.4% of wild-type | Paenibacillus sp. |
D607N | activity is completely abolished | Paenibacillus sp. |
E608D | Km value is decreased to about 57% and the kcat value is decreased to about 5.2% of wild-type | Paenibacillus sp. |
E608Q | Km value is decreased to about 26% and the kcat value is decreased to about 1.1% of wild-type | Paenibacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, strong inhibition | Paenibacillus sp. | |
Hg2+ | 5 mM, strong inhibition | Paenibacillus sp. | |
N-acetylgalactosaminide thiazoline | competitive | Paenibacillus sp. | |
N-acetylglucosaminide thiazoline | - |
Paenibacillus sp. | |
Ni2+ | 5 mM, strong inhibition | Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant E608Q, pH 6.0, 37°C | Paenibacillus sp. | |
0.2 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant E608D, pH 6.0, 37°C | Paenibacillus sp. | |
0.35 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | pH 6.0, 37°C | Paenibacillus sp. | |
1.74 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant D607E, pH 6.0, 37°C | Paenibacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
x * 115000, SDS-PAGE | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | E9RH20 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(GalNAcbeta(1-4))n + H2O = (GalNAcbeta(1-4))n-1 + 2-(acetylamino)-2-deoxy-beta-D-galactopyranose | NgaP adopts substrate-assisted catalysis in which residues Glu608 and Asp607 could function as a proton donor and a stabilizer of the 2-acetamide group of the beta-GalNAc at the active site, respectively | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-N-acetylglucosaminide + H2O | 1-3% of the activity with 4-nitrophenyl beta-N-acetylgalactosaminide | Paenibacillus sp. | beta-N-acetylglucosamine + 4-nitrophenol | - |
? | |
4-nitrophenyl N-acetyl-beta-D-galactosaminopyranoside + H2O | - |
Paenibacillus sp. | N-acetyl-D-galactosamine + 4-nitrophenol | - |
? | |
GalNAc-beta-1,3-Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-Cer + H2O | i.e. globotetraosylceramide, 39% of the activity with GalNAc-1,4-Gal-1,4-Glc-1,1-Cer | Paenibacillus sp. | GalNAc + Gal-alpha-1,4-Gal-beta-1,4-Glc-beta-1,1-Cer | - |
? | |
GalNAc-beta-1,4-Gal-beta-1,4-Glc-beta-1,1-Cer | i.e. asialo-GM2 | Paenibacillus sp. | GalNAc + Gal-beta-1,4-Glc-beta-1,1-Cer | - |
? | |
additional information | enzyme cleaves the nonreducing terminal beta-GalNAc residues of gangliotriaosylceramide and globotetraosylceramide. Enzyme prefers the GalNAc-1,4-linkage over the GalNAc-1,3-linkage | Paenibacillus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 115000, SDS-PAGE | Paenibacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
NgaP | - |
Paenibacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant E608Q, pH 6.0, 37°C | Paenibacillus sp. | |
0.1 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant D607E, pH 6.0, 37°C | Paenibacillus sp. | |
0.38 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant E608D, pH 6.0, 37°C | Paenibacillus sp. | |
7.3 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | wild-type, pH 6.0, 37°C | Paenibacillus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000013 | - |
N-acetylgalactosaminide thiazoline | pH 6.0, 37°C | Paenibacillus sp. | |
0.0468 | - |
N-acetylglucosaminide thiazoline | pH 6.0, 37°C | Paenibacillus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant D607E, pH 6.0, 37°C | Paenibacillus sp. | |
0.88 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant E608Q, pH 6.0, 37°C | Paenibacillus sp. | |
1.9 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | mutant E608D, pH 6.0, 37°C | Paenibacillus sp. | |
20.9 | - |
4-nitrophenyl beta-N-acetylgalactosaminide | pH 6.0, 37°C | Paenibacillus sp. |