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Literature summary for 3.2.1.53 extracted from

  • Tanaka, A.; Ozaki, S.
    Purification and characterization of beta-N-acetylgalactosaminidase from Bacillus sp. AT173-1 (1997), J. Biochem., 122, 330-336.
    View publication on PubMed
Search for more literature for 3.2.1.53

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ enzyme activity completely inhibited in the presence of 10 mM CuSO4 Bacillus sp. (in: Bacteria)
Fe2+ enzyme activity completely inhibited in the presence of 10 mM FeSO4 Bacillus sp. (in: Bacteria)
N-acetylgalactosamine relative activity in presence of 10 mM 50%, in presence of 50 mM 12% Bacillus sp. (in: Bacteria)
Urea relative activity in presence of 2 M 32% Bacillus sp. (in: Bacteria)
Zn2+ enzyme activity completely inhibited in the presence of 10 mM ZnSO4 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 4-nitrophenyl beta-acetylgalactosaminide
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ relative activity in presence of MgSO4 10 mM 104% Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 SDS-PAGE, enzyme denatured with SDS and 2-mercaptoethanol Bacillus sp. (in: Bacteria)
54000
-
 native enzyme, gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-N-acetylgalactosaminylated oligosaccharides + H2O Bacillus sp. (in: Bacteria)
-
 ?
-
 ?
beta-N-acetylgalactosaminylated oligosaccharides + H2O Bacillus sp. (in: Bacteria) AT173-1
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 AT173-1
-
Bacillus sp. (in: Bacteria) AT173-1
-
 AT173-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium AT 173-1, culture broth centrifuged to remove cells Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.4
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-acetylgalactosaminide + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + beta-acetylgalactosamine
-
 ?
4-nitrophenyl beta-acetylgalactosaminide + H2O
-
 Bacillus sp. (in: Bacteria) AT173-1 4-nitrophenol + beta-acetylgalactosamine
-
 ?
beta-N-acetylgalactosaminylated oligosaccharides + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
beta-N-acetylgalactosaminylated oligosaccharides + H2O
-
 Bacillus sp. (in: Bacteria) AT173-1 ?
-
 ?
N-acetyl-beta-D-galactosaminides + H2O substrate specificity towards beta-GalNAc residues is markedly higher than those of any other beta-GalNacase Bacillus sp. (in: Bacteria) N-acetyl-D-galactosamine + an alcohol
-
 ?
N-acetyl-beta-D-galactosaminides + H2O substrate specificity towards beta-GalNAc residues is markedly higher than those of any other beta-GalNacase Bacillus sp. (in: Bacteria) AT173-1 N-acetyl-D-galactosamine + an alcohol
-
 ?
p-nitrophenyl beta-N-acetylgalactosaminide + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
p-nitrophenyl beta-N-acetylgalactosaminide + H2O
-
 Bacillus sp. (in: Bacteria) AT173-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
Bacillus beta-GlcNAcase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 45 enzyme gradually inactivated in solution at 4°C, reactivated by incubation with DTT Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 4-methylumbelliferyl beta-N-acetylgalactosaminide Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 enzyme retains more than 80% activity Bacillus sp. (in: Bacteria)