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Literature summary for 3.2.1.52 extracted from

  • Kurakake, M.; Amai, Y.; Konishi, M.; Ikehira, K.
    Characteristics of an beta-N-acetylhexosaminidase from Bacillus sp. CH11, including its transglycosylation activity (2018), J. Food Sci., 83, 1208-1214 .
    View publication on PubMed
Search for more literature for 3.2.1.52

Organism

Organism UniProt Comment Textmining
Bacillus sp. CH11
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Purification (Commentary)

Purification (Comment) Organism
Super Q Toyopearl column chromatography Bacillus sp. CH11

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O 17% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide Bacillus sp. CH11 4-nitrophenol + N-acetyl-beta-D-galactosamine
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 ?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O 100% activity Bacillus sp. CH11 4-nitrophenol + N-acetyl-beta-D-glucosamine
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 ?
additional information no activity with 4-nitrophenyl beta-D-glucosaminide and 4-nitrophenyl beta-D-galactosaminide Bacillus sp. CH11 ?
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 ?
additional information transglycosylation to 1-propanol, 2-propanol, n-butanol, sorbitol, mannitol, L-arabinose, D-xylose, D-glucose, D-galactose, D-mannose, D-fructose, palatinose, trehalose, maltose, sucrose, lactose, raffinose, hesperidin, p-coumaric acid, o-coumaric acid, 3,4-dihydroxycinnamic acid, hydroxycinnamic acid, ferulic acid, gallic acid, quercetin is not observed Bacillus sp. CH11 ?
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 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
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 Bacillus sp. CH11 N-acetyl-beta-D-glucosamine + N,N',N'',N''',N''''pentaacetylchitopentaose + N,N',N'',N'''-tetraacetylchitotetraose
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 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
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 Bacillus sp. CH11 N-acetyl-beta-D-glucosamine + N,N',N'',N'''-tetraacetylchitotetraose + N,N',N''-triacetylchitotriose
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 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
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 Bacillus sp. CH11 N-acetyl-beta-D-glucosamine + N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose
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 ?
N,N',N''-triacetylchitotriose + H2O
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 Bacillus sp. CH11 N-acetyl-beta-D-glucosamine + N,N'-diacetylchitobiose
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 ?
N,N'-diacetylchitobiose + 1,3-propanediol
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + 1,4-butanediol
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + 1,5-pentanediol
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + erythritol
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + ethanol
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + ethylene glycol
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + glycerin
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + H2O
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 Bacillus sp. CH11 N-acetyl-beta-D-glucosamine
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 ?
N,N'-diacetylchitobiose + L-fucose
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + methanol
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 Bacillus sp. CH11 ?
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 ?
N,N'-diacetylchitobiose + xylitol
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 Bacillus sp. CH11 1-O-beta-D-N-acetylglucosaminyl xylitol
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 ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Bacillus sp. CH11

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
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-
 Bacillus sp. CH11

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 the enzyme activity is stable at temperatures of up to 40°C at pH 7.0 for 30 min Bacillus sp. CH11

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
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-
 Bacillus sp. CH11

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 the enzyme is stable from pH 6.0 to 8.0 at 40°C for 60 min Bacillus sp. CH11