Cloned (Comment) | Organism |
---|---|
gene cbsA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Thermotoga neapolitana |
gene nagA, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Thermotoga maritima MSB8 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 | |
0.29 | - |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 | |
0.3 | - |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana | |
0.36 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana | |
0.37 | - |
4-nitrophenyl di-N-acetyl-beta-D-chitobiose | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 | |
0.44 | - |
4-nitrophenyl di-N-acetyl-beta-D-chitobiose | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, recombinant enzyme, SDS-PAGE | Thermotoga neapolitana |
54000 | - |
2 * 54000, recombinant enzyme, SDS-PAGE | Thermotoga maritima MSB8 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima MSB8 | Q9WZR6 | gene nagA | - |
Thermotoga neapolitana | - |
gene cbsA | - |
Thermotoga neapolitana KCCM 41025 | - |
gene cbsA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography and gel filtration | Thermotoga neapolitana |
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography and gel filtration | Thermotoga maritima MSB8 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl di-N-acetyl-beta-D-chitobiose + H2O | NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga neapolitana | 4-nitrophenol + N-acetylglucosamine | - |
? | |
4-nitrophenyl di-N-acetyl-beta-D-chitobiose + H2O | NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga maritima MSB8 | 4-nitrophenol + N-acetylglucosamine | - |
? | |
4-nitrophenyl di-N-acetyl-beta-D-chitobiose + H2O | NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga neapolitana KCCM 41025 | 4-nitrophenol + N-acetylglucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | CbsA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga neapolitana | 4-nitrophenol + N-acetylglucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga maritima MSB8 | 4-nitrophenol + N-acetylglucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | CbsA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga neapolitana KCCM 41025 | 4-nitrophenol + N-acetylglucosamine | - |
? | |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose + H2O | NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga neapolitana | 4-nitrophenol + N-acetylglucosamine | - |
? | |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose + H2O | NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga maritima MSB8 | 4-nitrophenol + N-acetylglucosamine | - |
? | |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose + H2O | NagA initially hydrolyzes 4-nitrophenyl substrates to give GlcNAc | Thermotoga neapolitana KCCM 41025 | 4-nitrophenol + N-acetylglucosamine | - |
? | |
di-N-acetyl-beta-D-chitobiose + H2O | - |
Thermotoga neapolitana | N-acetylglucosamine | - |
? | |
di-N-acetyl-beta-D-chitobiose + H2O | - |
Thermotoga maritima MSB8 | N-acetylglucosamine | - |
? | |
di-N-acetyl-beta-D-chitobiose + H2O | - |
Thermotoga neapolitana KCCM 41025 | N-acetylglucosamine | - |
? | |
additional information | the enzyme shows exo-activity with chitin oligosaccharides from the non-reducing ends, no activity with 4-nitrophenyl-beta-D-galactose and 4-nitrophenyl N-acetyl-beta-D-galactosamine, or highly polymerized chitin | Thermotoga neapolitana | ? | - |
? | |
additional information | the enzyme shows exo-activity with chitin oligosaccharides from the non-reducing ends, no activity with 4-nitrophenyl-beta-D-galactose and 4-nitrophenyl N-acetyl-beta-D-galactosamine, or highly polymerized chitin | Thermotoga maritima MSB8 | ? | - |
? | |
additional information | the enzyme shows exo-activity with chitin oligosaccharides from the non-reducing ends, no activity with 4-nitrophenyl-beta-D-galactose and 4-nitrophenyl N-acetyl-beta-D-galactosamine, or highly polymerized chitin | Thermotoga neapolitana KCCM 41025 | ? | - |
? | |
tri-N-acetyl-beta-D-chitotriose + H2O | - |
Thermotoga neapolitana | N-acetylglucosamine | - |
? | |
tri-N-acetyl-beta-D-chitotriose + H2O | - |
Thermotoga maritima MSB8 | N-acetylglucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, recombinant enzyme, SDS-PAGE | Thermotoga neapolitana |
dimer | 2 * 54000, recombinant enzyme, SDS-PAGE | Thermotoga maritima MSB8 |
Synonyms | Comment | Organism |
---|---|---|
beta-N-acetylglucosaminidase | - |
Thermotoga neapolitana |
beta-N-acetylglucosaminidase | - |
Thermotoga maritima MSB8 |
CbsA | - |
Thermotoga neapolitana |
More | the enzyme belongs to the glycosyl hydrolase family 3, GH3 | Thermotoga neapolitana |
More | the enzyme belongs to the glycosyl hydrolase family 3, GH3 | Thermotoga maritima MSB8 |
NagA | - |
Thermotoga maritima MSB8 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermotoga maritima MSB8 |
75 | - |
- |
Thermotoga neapolitana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 95 | activity profile, over 65% of maximal activity at 95°C, overview | Thermotoga neapolitana |
50 | 95 | activity profile, over 65% of maximal activity at 95°C, overview | Thermotoga maritima MSB8 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
146.7 | - |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 | |
147.2 | - |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana | |
199 | - |
4-nitrophenyl di-N-acetyl-beta-D-chitobiose | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 | |
220.8 | - |
4-nitrophenyl di-N-acetyl-beta-D-chitobiose | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana | |
246.2 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 | |
273.4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Thermotoga neapolitana |
7 | 8 | - |
Thermotoga maritima MSB8 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | over 80% of maximal activity within the range of pH 6.5-8.5, rapid decline over pH 9.0, activity profile, overview | Thermotoga neapolitana |
4.5 | 10 | over 80% of maximal activity within the range of pH 6.5-8.5, rapid decline over pH 9.0, activity profile, overview | Thermotoga maritima MSB8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
490.7 | - |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana | |
501.2 | - |
4-nitrophenyl di-N-acetyl-beta-D-chitobiose | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana | |
505.7 | - |
4-nitrophenyl tri-N-acetyl-beta-D-chitotriose | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 | |
538 | - |
4-nitrophenyl di-N-acetyl-beta-D-chitobiose | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 | |
759.4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | pH 7.5, 70°C, recombinant enzyme | Thermotoga neapolitana | |
879.3 | - |
4-nitrophenyl N-acetyl-beta-D-glucosamine | pH 7.5, 70°C, recombinant enzyme | Thermotoga maritima MSB8 |