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Literature summary for 3.2.1.52 extracted from

  • Macauley, M.S.; Whitworth, G.E.; Debowski, A.W.; Chin, D.; Vocadlo, D.J.
    O-GlcNAcase uses substrate-assisted catalysis: kinetic analysis and development of highly selective mechanism-inspired inhibitors (2005), J. Biol. Chem., 280, 25313-25322.
    View publication on PubMed
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
1,2-dideoxy-2'-butyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
1,2-dideoxy-2'-ethyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
1,2-dideoxy-2'-isobutyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
1,2-dideoxy-2'-isopropyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
1,2-dideoxy-2'-methyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline an inhibitor that mimics the oxazoline intermediate proposed in the catalytic mechanism of family 20 glycoside hydrolases, is shown to act as a potent competitive inhibitor. About 17000fold more tightly than GlcNAc Homo sapiens
1,2-dideoxy-2'-pentyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
1,2-dideoxy-2'-propyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
GlcNAc
-
 Homo sapiens
additional information streptozotocin does not appear to act as an irreversible inhibitor Homo sapiens
O-(2-acetamido-2-deoxy-D-glucopyranosylidene) amino-N-phenylcarbamate PUGNAc Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
 4-methylcoumar-7-yl-beta-N-acetyl-D-glucosamine
-
 Homo sapiens
3.8
-
 4-methylcoumar-7-yl-beta-N-fluoroacetyl-D-glucosamine
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcoumar-7-yl-beta-N-acetyl-D-glucosamine + H2O
-
 Homo sapiens ?
-
 ?
4-methylcoumar-7-yl-beta-N-fluoroacetyl-D-glucosamine + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-hexosaminidase
-
 Homo sapiens
lysosomal beta-hexosaminidase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000036
-
 O-(2-acetamido-2-deoxy-D-glucopyranosylidene) amino-N-phenylcarbamate
-
 Homo sapiens
0.00007
-
 1,2-dideoxy-2'-methyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
0.032
-
 1,2-dideoxy-2'-ethyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
0.34
-
 1,2-dideoxy-2'-propyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
0.72
-
 1,2-dideoxy-2'-isopropyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
1.2
-
 GlcNAc
-
 Homo sapiens
4
-
 1,2-dideoxy-2'-isobutyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
4.6
-
 1,2-dideoxy-2'-butyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens
11
-
 1,2-dideoxy-2'-pentyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline
-
 Homo sapiens