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Literature summary for 3.2.1.52 extracted from

  • Aunpad, R.; Rice, D.W.; Sedelnikova, S.; Panbangred, W.
    Biochemical characterisation of two forms of halo- and thermo-tolerant chitinase C of Salinivibrio costicola expressed in Escherichia coli (2007), Ann. Microbiol., 57, 249-257.
No PubMed abstract available
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, Chi-I Salinivibrio costicola
expression in Escherichia coli, Chi-II Salinivibrio costicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 p-nitrophenyl N,N'-diacetylchitobiose Chi-I Salinivibrio costicola
0.0318
-
 p-nitrophenyl-N,N'-diacetylchitobiose Chi-II Salinivibrio costicola

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM, 10% enhancement of activity, Chi-I Salinivibrio costicola
Mg2+ 10 mM, 10% enhancement of activity, Chi-II Salinivibrio costicola
NaCl salinity optimum at 1-2% NaCl, retains more than 80% of its activity at 3-5% NaCl and more than 50% residual activity at 14% NaCl, The enzyme can also well function in absence of NaCl, Chi-I Salinivibrio costicola
NaCl salinity optimum at 1-2% NaCl, retains more than 80% of its activity at 3-5% NaCl and more than 50% residual activity at 14% NaCl, The enzyme can also well function in absence of NaCl, Chi-II Salinivibrio costicola

Organism

Organism UniProt Comment Textmining
Salinivibrio costicola
-
-
-
Salinivibrio costicola 5SM-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-N,N'-diacetylchitobiose + H2O Chi-I Salinivibrio costicola ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O Chi-II Salinivibrio costicola ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O Chi-I Salinivibrio costicola 5SM-1 ?
-
 ?
p-nitrophenyl-N,N'-diacetylchitobiose + H2O Chi-II Salinivibrio costicola 5SM-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
Chi-I
-
 Salinivibrio costicola
Chi-II Chi-II is derived from Chi-I by C-terminal processing Salinivibrio costicola
exochitinase
-
 Salinivibrio costicola

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 Chi-I Salinivibrio costicola
50
-
 Chi-II Salinivibrio costicola

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 60 min, less than 50% loss of activity, Chi-I Salinivibrio costicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 Chi-I Salinivibrio costicola
7
-
 Chi-II Salinivibrio costicola

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 stable pH-range, Chi-I Salinivibrio costicola
3 10 stable pH-ranhe, Chi-II Salinivibrio costicola