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Literature summary for 3.2.1.52 extracted from
- beta-Hexosaminidase, an enzyme from ripening bell capsicum (Capsicum annuum var variata) (2002), Phytochemistry, 61, 295-300.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, activation of isoenzyme I to 115.2% of control | Capsicum annuum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 16% inhibition of isoenzyme II, slight activation of isoenzyme I | Capsicum annuum | |
| Cu2+ | 1 mM, 91% inhibition of isoenzyme I, 22% inhibition of isoenzyme II | Capsicum annuum | |
| Hg2+ | 1 mM, 97% inhibition of isoenzyme I, 95% inhibition of isoenzyme II | Capsicum annuum | |
| Mg2+ | 1 mM, 33% inhibition of isoenzyme II, slight activation of isoenzyme I | Capsicum annuum | |
| Zn2+ | 1 mM, 86% inhibition of isoenzyme I, 62% inhibition of isoenzyme II | Capsicum annuum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.75 | - |
p-nitrophenyl-N-acetyl-beta-D-glucosaminide | isoenzyme II | Capsicum annuum | |
| 3 | - |
p-nitrophenyl-N-acetyl-beta-D-glucosaminide | isoenzyme I | Capsicum annuum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, activation of isoenzyme I to 110.8% of control | Capsicum annuum | |
| Mg2+ | 1 mM, activation of isoenzyme I to 109.5% of control | Capsicum annuum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
1 * 85000, isoenzyme II, SDS-PAGE | Capsicum annuum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Capsicum annuum | - |
var. variata | - |
| Carica papaya | - |
- |
- |
| Mangifera indica | - |
- |
- |
| Musca sp. | - |
banana | - |
| Solanum lycopersicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoform I and II | Capsicum annuum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Solanum lycopersicum | - |
| fruit | - |
Capsicum annuum | - |
| fruit | - |
Carica papaya | - |
| fruit | - |
Mangifera indica | - |
| fruit | - |
Musca sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.31 | - |
isoenzyme II | Capsicum annuum |
| 10.11 | - |
isoenzyme I | Capsicum annuum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Capsicum annuum | p-nitrophenol + beta-D-N-acetyl-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 85000, isoenzyme II, SDS-PAGE | Capsicum annuum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-hexosaminidase I | - |
Capsicum annuum |
| beta-hexosaminidase II | - |
Capsicum annuum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 47 | isoenzyme I | Capsicum annuum |
| 47 | - |
isoenzyme II | Capsicum annuum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
15 min, isoenzyme II is stable, isoenzyme I loses 30% of the initial activity | Capsicum annuum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
isoenzyme I | Capsicum annuum |
| 5 | - |
isoenzyme II | Capsicum annuum |
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Release 2023.1 (January 2023)
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