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Literature summary for 3.2.1.52 extracted from

  • Oikawa, A.; Itoh, E.; Ishihara, A.; Iwamura, H.
    Purification and characterization of beta-N-acetylhexosaminidase from maize seedlings (2003), J. Plant Physiol., 160, 991-999.
    View publication on PubMed
Search for more literature for 3.2.1.52

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 5 mM, 93% inhibition Zea mays
CuCl2 5 mM, 37% inhibition Zea mays
FeCl3 5 mM, 90% inhibition Zea mays
HgCl2 5 mM, 96% inhibition Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
AlCl3 5 mM, activation to 124% Zea mays
CaCl2 5 mM, activation to 116% Zea mays
KCl 5 mM, activation to 111% Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 gel filtration Zea mays
70000
-
 1 * 70000, SDS-PAGE Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
 L. cv. Snowdent 110
-

Purification (Commentary)

Purification (Comment) Organism
-
 Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Zea mays
-
scutellum
-
 Zea mays
-
seed substantial activity detected in dry seed. Activity increases before germination but exclusively in the embryo Zea mays
-
seedling most of the activity is found in the scutellum, and lower levels in shoots and roots Zea mays
-
shoot
-
 Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O release of GlcNAc after 24 h Zea mays GlcNAc + ?
-
 ?
additional information no activity with oligo-sugar I, oligo-sugar II and ovalbumin Zea mays ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Zea mays N-acetyl-D-glucosamine + ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Zea mays N-acetyl-D-glucosamine + ?
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Zea mays N-acetyl-D-glucosamine + N,N'-diacetylchiotobiose
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Zea mays deacetylated chitooligosaccharides + acetate
-
 ?
p-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O 7.39% of the activity with (GlcNAc)2 Zea mays p-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O 5.95% of the activity with (GlcNAc)2 Zea mays p-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 reaction with (GlcNAc)2 Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 50% of maximal activcity at pH 3.5 and pH 6.0 Zea mays

pI Value

Organism Comment pI Value Maximum pI Value
Zea mays chromatofocusing
-
 6