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Literature summary for 3.2.1.52 extracted from

  • Kurakake, M.; Goto, T.; Ashiki, K.; Suenaga, Y.; Komaki, T.
    Synthesis of new glycosides by transglycosylation of N-acetylhexosaminidase from Serratia marcescens YS-1 (2003), J. Agric. Food Chem., 51, 1701-1705.
    View publication on PubMed
Search for more literature for 3.2.1.52

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 x * 95000, SDS-PAGE Serratia marcescens

Organism

Organism UniProt Comment Textmining
Serratia marcescens
-
 YS-1
-
Serratia marcescens Ys-1
-
 YS-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Serratia marcescens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture liquid culture Serratia marcescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.36
-
-
 Serratia marcescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
di-N-acetylchitobiose + 1,2-butanediol transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 1,2-butanediol transglycosylation Serratia marcescens Ys-1 ?
-
 ?
di-N-acetylchitobiose + 1,2-ethanediol transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 1,2-ethanediol transglycosylation Serratia marcescens Ys-1 ?
-
 ?
di-N-acetylchitobiose + 1,2-propanediol transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 1,2-propanediol transglycosylation Serratia marcescens Ys-1 ?
-
 ?
di-N-acetylchitobiose + 1,3-propanediol transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 1,3-propanediol transglycosylation Serratia marcescens Ys-1 ?
-
 ?
di-N-acetylchitobiose + 1,4-butanediol transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 1,5-pentanediol transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 1,6-hexanediol transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 1-butanol transglycosylation. The N-acetylglucosamine residue is transferred to the hydroxyl group at C1 of monoalcohols Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 1-propanol transglycosylation. The N-acetylglucosamine residue is transferred to the hydroxyl group at C1 of monoalcohols Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 2-butanol transglycosylation. The N-acetylglucosamine residue is transferred to the hydroxyl group at C1 of monoalcohols Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 2-methyl-1-propanol transglycosylation. The N-acetylglucosamine residue is transferred to the hydroxyl group at C1 of monoalcohols Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 2-methyl-2-propanol transglycosylation. The N-acetylglucosamine residue is transferred to the hydroxyl group at C1 of monoalcohols Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + 2-propanol transglycosylation. The N-acetylglucosamine residue is transferred to the hydroxyl group at C1 of monoalcohols Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + arabitol weak transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + dulcitol weak transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + ethanol transglycosylation. The N-acetylglucosamine residue is transferred to the hydroxyl group at C1 of monoalcohols Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + H2O
-
 Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + lactitol weak transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + mannitol weak transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + methanol transglycosylation Serratia marcescens methyl N-acetylglucosaminide + tri-N-acetylchitotriose + ?
-
 ?
di-N-acetylchitobiose + myoinositol weak transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + palatinitol weak transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + sorbitol weak transglycosylation Serratia marcescens ?
-
 ?
di-N-acetylchitobiose + xylitol weak transglycosylation Serratia marcescens ?
-
 ?
additional information no activity with cellobiose and chitobiose. No transglycosylation with 1,3-butanediol, 2,3-butanediol and 1,2,4-butanetriol Serratia marcescens ?
-
 ?
additional information no activity with cellobiose and chitobiose. No transglycosylation with 1,3-butanediol, 2,3-butanediol and 1,2,4-butanetriol Serratia marcescens Ys-1 ?
-
 ?
p-nitrophenyl-N-acetyl-alpha-D-galactosaminide + H2O 1.5% of the activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide Serratia marcescens p-nitrophenol + N-acetyl-alpha-D-galactosamine
-
 ?
p-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O 28.1% of the activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide Serratia marcescens p-nitrophenol + N-acetyl-beta-D-galactosamine
-
 ?
p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Serratia marcescens p-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O 2.2% of the activity with p-nitrophenyl-N-acetyl-beta-D-glucosaminide Serratia marcescens p-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
tetra-N-acetylchitotetraose + H2O
-
 Serratia marcescens ?
-
 ?
tri-N-acetylchitotriose + H2O
-
 Serratia marcescens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 95000, SDS-PAGE Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 h, pH 6-8, stable Serratia marcescens
50
-
 pH 5, 30 min, relatively stable below Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 transglycosylation of di-N-acetylchitobiose and methanol Serratia marcescens
8 9
-
 Serratia marcescens