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Literature summary for 3.2.1.52 extracted from

  • Trimble, R.B.; Tarentino, A.L.; Evans Aumick, G.; Maley, F.
    endo-beta-N-Acetylglucosaminidase L from Streptomyces plicatus (1982), Methods Enzymol., 83, 603-610.
    View publication on PubMed
Search for more literature for 3.2.1.52

General Stability

General Stability Organism
stable to repeated freeze-thaw cycles and to lyophilization, 20-30% of activity are lost if enzyme concentration is decreased to 0.02 mg/ml, stable with 0.1 mg bovine serum albumin per ml Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 (N-acetyl-beta-D-glucosamine)3
-
 Streptomyces sp.
0.7
-
 dansyl-Asn(GlcNAc)2Man
-
 Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 gel filtration Streptomyces sp.
49500
-
 sedimentation equilibrium Streptomyces sp.
52000
-
 SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Streptomyces sp.
-
 N-acetyl-D-hexosamines
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
sodium dodecyl sulfate complete inactivation with 0.2% in less than 2 h at 37°C Streptomyces sp.
Triton X-100 loss of 70% of activity with 0.2% after 5 h at 37°C Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 S. plicatus
-
Streptomyces sp.
-
 ATCC 27800
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Streptomyces sp.

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose endo-type reaction Streptomyces sp.
a

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.97
-
 pool III Streptomyces sp.
1
-
 pool I Streptomyces sp.
1.05
-
 pool II Streptomyces sp.

Storage Stability

Storage Stability Organism
4°C, crude supernatant fraction stable for at least a year Streptomyces sp.
4°C, purified enzyme after sterilization stable for at least 6 months Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(GlcNAc)2Man + H2O
-
 Streptomyces sp. ?
-
 ?
(N-acetyl-beta-D-glucosamine)3 + H2O
-
 Streptomyces sp. (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
 ?
Asn(GlcNAc)2Man + H2O
-
 Streptomyces sp. ?
-
 ?
chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Streptomyces sp. N-acetyl-D-glucosamine
-
 ?
dansyl-Asn(GlcNAc)2 + H2O
-
 Streptomyces sp. ?
-
 ?
dansyl-Asn(GlcNAc)2Man + H2O
-
 Streptomyces sp. dansyl-AsnGlcNAc + ?
-
 ?
dansyl-Asn(GlcNAc)2Man2 + H2O
-
 Streptomyces sp. ?
-
 ?
GlcNAC-beta-(1->4)-GlcNAC-beta-(1->4)-GlcNAC-beta-(1->4)-GlcNAc-ol + H2O
-
 Streptomyces sp. ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Streptomyces sp. 2 N,N-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Streptomyces sp. N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Streptomyces sp. deacetylated chitooligosaccharides + acetate
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Streptomyces sp. N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Streptomyces sp. 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase
-
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 pH from 3.0-8.0, stable for 24 h Streptomyces sp.
25
-
 pH from 3.0-8.0, stable for 24 h Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5 substrate: dansyl-Asn(GlcNAc)2Man Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 half-maximal activity Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
 half-maximal activity Streptomyces sp.