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Literature summary for 3.2.1.52 extracted from

  • Khar, A.; Anand, S.R.
    Studies on the glycosidases of semen. Purification and properties of beta-N-acetylglucosaminidase from bull sperm (1977), Biochim. Biophys. Acta, 483, 141-151.
    View publication on PubMed
Search for more literature for 3.2.1.52

Activating Compound

Activating Compound Comment Organism Structure
cysteine
-
 Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
acetate
-
 Bos taurus
Ag+
-
 Bos taurus
galactonolactone
-
 Bos taurus
galactosamine
-
 Bos taurus
gluconolactone
-
 Bos taurus
glucosamine
-
 Bos taurus
Hg2+
-
 Bos taurus
iodoacetamide
-
 Bos taurus
N-acetyl-D-galactosamine
-
 Bos taurus
N-acetyl-D-glucosamine
-
 Bos taurus
p-chloromercuribenzoate
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Bos taurus
0.52
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
acrosome
-
 Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13400
-
 1 * 53000 + 1 * 13400, SDS-PAGE Bos taurus
53000
-
 1 * 53000 + 1 * 13400, SDS-PAGE Bos taurus
190000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Bos taurus
-
 N-acetyl-D-hexosamines
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
2-mercaptoethanol denaturation in subunits Bos taurus
guanidine-HCl denaturation in subunits Bos taurus
urea denaturation in subunits Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Bos taurus

Purification (Commentary)

Purification (Comment) Organism
135fold to homogeneity Bos taurus

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose seperate active sites for glucosaminidase and galactosaminidase activities Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
semen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.63
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha1-acid glycoprotein + H2O
-
 Bos taurus ?
-
 ?
chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-4 Bos taurus N-acetyl-D-glucosamine
-
 ?
kappa-casein glycopeptide + H2O
-
 Bos taurus ?
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Bos taurus N-acetyl-D-hexosamines
-
 ?
ovalbumin glycopeptides + H2O
-
 Bos taurus ?
-
 ?
ovomucoid glycopeptide + H2O
-
 Bos taurus ?
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bos taurus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 53000 + 1 * 13400, SDS-PAGE Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable for 60 min at pH 4.5 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4
-
 N-acetylgalactosamnidase activity Bos taurus
4.5
-
 N-acetylglucosamnidase activity Bos taurus