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Literature summary for 3.2.1.52 extracted from
- Studies on Turbatrix aceti beta-N-acetylglucosaminidase. 1.Purification and physicochemical characterization (1984), Arch. Biochem. Biophys., 233, 237-250.
General Stability
| General Stability | Organism |
|---|---|
| unstable to freezing and thawing | Turbatrix aceti |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | concentration of 1 mM causes 25% inhibition | Turbatrix aceti |  |
| acetate | concentration of 1 mM causes 30% inhibition | Turbatrix aceti | |
| EDTA | concentration of 1 mM causes 40% inhibition | Turbatrix aceti | |
| Fe3+ | concentration of 1 mM causes 70-80% inhibition | Turbatrix aceti | |
| Hg2+ | concentration of 1 mM causes complete inhibition | Turbatrix aceti | |
| p-nitrophenyl 2-deoxy-2-thioacetamido-beta-D-galactopyranoside | competitive inhibition | Turbatrix aceti | |
| p-nitrophenyl 2-deoxy-2-thioacetamido-beta-D-glucopyranoside | competitive inhibition | Turbatrix aceti | |
| Zn2+ | concentration of 1 mM causes 70-80% inhibition | Turbatrix aceti | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Turbatrix aceti | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 112000 | - |
native PAGE | Turbatrix aceti |
| 124000 | - |
gel filtration | Turbatrix aceti |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Turbatrix aceti | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Turbatrix aceti | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | mannosylation | Turbatrix aceti |
| glycoprotein | galactosylation | Turbatrix aceti |
| glycoprotein | N-acetyl-glucosaminylation | Turbatrix aceti |
| glycoprotein | fucosylation | Turbatrix aceti |
| glycoprotein | N-acetyl-galactosaminylation | Turbatrix aceti |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 1200fold | Turbatrix aceti |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose | exo-type reaction | Turbatrix aceti |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Turbatrix aceti | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 90 | - |
substrate: phenyl-N-acetyl-beta-D-glucosaminide | Turbatrix aceti |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, crude culture supernatant | Turbatrix aceti |
| 4°C, purified enzyme, stable for several months in presence of 30% (NH4)2SO4 in 50 mM phosphate buffer, pH 7.0 | Turbatrix aceti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| asialo-agalactofetuin + H2O | - |
Turbatrix aceti | ? | - |
? | |
| chitin oligosaccharides + H2O | - |
Turbatrix aceti | N-acetyl-D-glucosamine | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Turbatrix aceti | N-acetyl-D-hexosamines | - |
? | |
| ovalbumin + H2O | - |
Turbatrix aceti | N-acetylglucosamine + ? | - |
? | |
| p-nitrophenyl 2-deoxy-2-thioacetamido-beta-D-galactopyranoside + H2O | poor substrate | Turbatrix aceti | 4-nitrophenol + 2-deoxy-2-thioacetamido-beta-D-galactopyranoside | - |
? | |
| p-nitrophenyl 2-deoxy-2-thioacetamido-beta-D-glucopyranoside + H2O | poor substrate | Turbatrix aceti | 4-nitrophenol + 2-deoxy-2-thioacetamido-beta-D-glucopyranoside | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Turbatrix aceti | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Turbatrix aceti | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
| phenyl-N-acetyl-beta-D-glucosaminide + H2O | - |
Turbatrix aceti | phenol + N-acetyl-beta-D-glucosaminide | - |
? | |
| Smith degradation product of human salivary glycoprotein oligosaccharide + H2O | - |
Turbatrix aceti | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | major band: 68900, minor band: 65900, SDS-PAGE | Turbatrix aceti |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
N-acetylglucosamnidase activity | Turbatrix aceti |
| 5.4 | - |
N-acetylgalactosaminidase activity | Turbatrix aceti |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
sharp decline of N-acetylglucosaminidase activity on either side of this value | Turbatrix aceti |
| 5.4 | - |
sharp decline of N-acetylgalactosaminidase activity on either side of this value | Turbatrix aceti |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
stable around pH 7.0 | Turbatrix aceti |
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