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Literature summary for 3.2.1.52 extracted from

  • Koga, D.; Nakashima, M.; Matsukara, T.; Kimura, S.; Ide, A.
    Purification and properties of beta-N-acetyl-D-glucosaminidase from alimentary canal of the silkworm, Bombyx mori (1986), Agric. Biol. Chem., 50, 2357-2368.
No PubMed abstract available
Search for more literature for 3.2.1.52

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.0075 mM: activity reaches 110.0% Bombyx mori

General Stability

General Stability Organism
lyophilization reduces activity to 34.5% Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ with 0.03 mM 42.9% of activity remains Bombyx mori
Hg2+ with 0.03 mM 7.1% of activity remains Bombyx mori
Mg2+ with 0.03 mM 71.4% of activity remains Bombyx mori
additional information high substrate concentrations, i.e. concentrations above 0.2 mM Bombyx mori
Zn2+ with 0.03 mM 88.6% of activity remains Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 (N-acetyl-beta-D-glucosamine)6
-
 Bombyx mori
0.09
-
 (N-acetyl-beta-D-glucosamine)5
-
 Bombyx mori
0.118
-
 (N-acetyl-beta-D-glucosamine)4
-
 Bombyx mori
0.122
-
 (N-acetyl-beta-D-glucosamine)3
-
 Bombyx mori
0.397
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Bombyx mori
0.433
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Bombyx mori
0.787
-
 (N-acetyl-beta-D-glucosamine)2
-
 Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
 alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
67500
-
 alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
67500
-
 Ex 1, homodimer: 2 * 67500 Bombyx mori
119000
-
 gel filtration Bombyx mori
125000
-
 SDS-PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Bombyx mori
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
 5-day old pupae
-

Purification (Commentary)

Purification (Comment) Organism
64fold to homogeneity Bombyx mori

Reaction

Reaction Comment Organism Reaction ID
(N-acetyl-D-glucosamine)4 + 3 H2O = 4 2-(acetylamino)-2-deoxy-beta-D-glucopyranose exo-type reaction Bombyx mori
a

Source Tissue

Source Tissue Comment Organism Textmining
alimentary canal
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60.6
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Bombyx mori

Storage Stability

Storage Stability Organism
-20°C, after 27 days 87.1% of activity remains Bombyx mori
4°C, not stable, rapid decrease of activity after the first 12 days Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(N-acetyl-beta-D-glucosamine)2 + H2O
-
 Bombyx mori 2 N-acetyl-beta-D-glucosamine
-
 ?
(N-acetyl-beta-D-glucosamine)3 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)2 + N-acetyl-beta-D-glucosamine
-
 ?
(N-acetyl-beta-D-glucosamine)4 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)3 + N-acetyl-beta-D-glucosamine
-
 ?
(N-acetyl-beta-D-glucosamine)5 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)4 + N-acetyl-beta-D-glucosamine
-
 ?
(N-acetyl-beta-D-glucosamine)6 + H2O
-
 Bombyx mori (N-acetyl-beta-D-glucosamine)5 + N-acetyl-beta-D-glucosamine
-
 ?
chitin + H2O colloidal chitin Bombyx mori N-acetylglucosamine
-
 ?
chitin oligosaccharides + H2O (N-acetyl-D-glucosamine)2-6 Bombyx mori N-acetyl-D-glucosamine
-
 ?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Bombyx mori ?
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Bombyx mori ?
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Bombyx mori 2 N,N-diacetylchitobiose
-
 ?
N,N',N''-triacetylchitotriose + H2O
-
 Bombyx mori N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Bombyx mori deacetylated chitooligosaccharides + acetate
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Bombyx mori N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Bombyx mori 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bombyx mori 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
dimer alimentary canal enzyme: 1 * 67500 + 1 * 57500, SDS-PAGE Bombyx mori
dimer Ex 1, homodimer: 2 * 67500 Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 alimentary canal enzyme Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable below, for 10 min Bombyx mori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
51.9
-
 (N-acetyl-beta-D-glucosamine)6
-
 Bombyx mori
75.6
-
 (N-acetyl-beta-D-glucosamine)5
-
 Bombyx mori
129
-
 (N-acetyl-beta-D-glucosamine)4
-
 Bombyx mori
209
-
 (N-acetyl-beta-D-glucosamine)3
-
 Bombyx mori
820
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
 Bombyx mori
1010
-
 (N-acetyl-beta-D-glucosamine)2
-
 Bombyx mori
1150
-
 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
 Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Bombyx mori

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 stable Bombyx mori