Any feedback?
Literature summary for 3.2.1.52 extracted from
- Properties of four molecular forms of N-acetyl-beta-D-hexosaminidase isolated from germinating seeds of fenugreek (Trigonella foenum graecum) (1978), Eur. J. Biochem., 84, 551-559.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | at 5% both hexosaminidase activities of forms II and I are increased 2- and 1.5fold | Trigonella foenum-graecum | |
| Fe2+ | stimulates both hexosaminidase activities of form I | Trigonella foenum-graecum |  |
| Fe3+ | stimulates galactosaminidase activitiy of form I | Trigonella foenum-graecum | |
| L-cysteine | concentrations of 0.05, 0.1, 0.4 and 0.7 mM are needed to reactivate both hexosaminidase activities of forms I, II, III and IV | Trigonella foenum-graecum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | all 4 forms | Trigonella foenum-graecum | |
| Fe2+ | both hexosaminidase activities of forms II, III and IV inhibited | Trigonella foenum-graecum | |
| Fe3+ | glucosaminidase-activity of forms II, III and IV are depressed, galactosaminidase-activity of forms III and IV are inhibited | Trigonella foenum-graecum | |
| Hg2+ | all 4 forms | Trigonella foenum-graecum | |
| p-chloromercuribenzoate | inhibits forms II, III and IV, competitive inhibition | Trigonella foenum-graecum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | KM 0.056, new form I, after 4 months of storage at 4°C | Trigonella foenum-graecum | |
| 0.04 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | form I | Trigonella foenum-graecum | |
| 0.04 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | form IV | Trigonella foenum-graecum | |
| 0.087 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | form III | Trigonella foenum-graecum | |
| 0.09 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | form III | Trigonella foenum-graecum | |
| 0.15 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | form I | Trigonella foenum-graecum | |
| 0.37 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | form IV | Trigonella foenum-graecum | |
| 0.55 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside | form II | Trigonella foenum-graecum | |
| 0.62 | - |
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | form II | Trigonella foenum-graecum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
form II, SDS-PAGE | Trigonella foenum-graecum |
| 28000 | - |
form I, SDS-PAGE | Trigonella foenum-graecum |
| 30000 | - |
form IV, SDS-PAGE | Trigonella foenum-graecum |
| 30500 | - |
form III, SDS-PAGE | Trigonella foenum-graecum |
| 62000 | - |
new form I, after 4 months of storage at 4°C | Trigonella foenum-graecum |
| 72000 | - |
form II, gel filtration | Trigonella foenum-graecum |
| 84000 | - |
form I, gel filtration | Trigonella foenum-graecum |
| 150000 | - |
form IV, gel filtration | Trigonella foenum-graecum |
| 180000 | - |
form III, gel filtration | Trigonella foenum-graecum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Trigonella foenum-graecum | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trigonella foenum-graecum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | mannosylation | Trigonella foenum-graecum |
| glycoprotein | N-acetyl-glucosaminylation | Trigonella foenum-graecum |
| glycoprotein | fucosylation | Trigonella foenum-graecum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| form I: 2262fold, form II: 2102fold, form III: 20380fold, form IV: 5061fold | Trigonella foenum-graecum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | forms I and II | Trigonella foenum-graecum | - |
| embryo | forms III and IV | Trigonella foenum-graecum | - |
| endosperm | forms I and II | Trigonella foenum-graecum | - |
| seed | germinating seeds | Trigonella foenum-graecum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.64 | - |
form II | Trigonella foenum-graecum |
| 2.82 | - |
form I | Trigonella foenum-graecum |
| 6.35 | - |
form IV | Trigonella foenum-graecum |
| 25.68 | - |
form III | Trigonella foenum-graecum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, after 2 months of storage in phosphate buffer pH 5.0 only form I shows decrease of activity by 50-60%, after 4 months a new form of enzyme with a molecular weight of 62000 Da and a Km of 0.056 mM appears | Trigonella foenum-graecum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O | - |
Trigonella foenum-graecum | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside | - |
? | |
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Trigonella foenum-graecum | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
| additional information | - |
Trigonella foenum-graecum | ? | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Trigonella foenum-graecum | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Trigonella foenum-graecum | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Trigonella foenum-graecum | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 30500, SDS-PAGE, form III | Trigonella foenum-graecum |
| pentamer | 5 * 30000, SDS-PAGE, form IV | Trigonella foenum-graecum |
| trimer | 3 * 28000, SDS-PAGE, form I | Trigonella foenum-graecum |
| trimer | 3 * 24000, SDS-PAGE, form II | Trigonella foenum-graecum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | all 4 forms | Trigonella foenum-graecum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
at its optimum pH both hexosaminidase activities of each of the 4 forms remain stable over 5 hours | Trigonella foenum-graecum |
| 50 | - |
both hexosaminidase activities of form I decrease rapidly, forms II, III and IV show biphasic denaturation curves | Trigonella foenum-graecum |
| 60 | - |
complete inactivation of all 4 forms after 20 min | Trigonella foenum-graecum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form III | Trigonella foenum-graecum |
| 4.6 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form IV | Trigonella foenum-graecum |
| 4.8 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form IV | Trigonella foenum-graecum |
| 5 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, forms II and III | Trigonella foenum-graecum |
| 5 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form II | Trigonella foenum-graecum |
| 5.5 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, form I | Trigonella foenum-graecum |
| 6 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form I | Trigonella foenum-graecum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
