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Literature summary for 3.2.1.52 extracted from
- Purification and some properties of liver and brain beta-N-acetyl-hexosaminidase S (1979), Biochim. Biophys. Acta, 566, 80-87.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-galactosamine | with 30 mM the activity of liver and brain enzymes reaches 135% and 114.3% | Homo sapiens |  |
| D-glucosamine | with 30 mM the activity of liver and brain enzymes reaches 135% and 110.7% | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetate | with 30 mM 39.8% of liver enzyme type S, 71.4% of brain enzyme type S, 33.3-39.4% of liver enzyme type A and 30.6-38.2% of liver enzyme type B remains | Homo sapiens | |
| galactosamine | with 30 mM 84.3-89.3% of liver enzyme type A and 79.6-87.8% of liver enzyme type B remains | Homo sapiens | |
| glucosamine | with 30 mM 72.2-76.3% of liver enzyme type A and 65.7-73.3% of liver enzyme type B remains | Homo sapiens | |
| N-acetyl-D-galactosamine | with 30 mM 12.1% activity of liver enzyme type S, 13.6% of brain enzyme type S, 3.2-8.6% of liver enzyme type A and 2.9-9.9% of liver enzyme type B remains | Homo sapiens | |
| N-acetyl-D-glucosamine | with 30 mM 40.7% activity of liver enzyme type S, 19.9% of brain enzyme type S, 19.9-24.6% of liver enzyme type A and 20.9-30.5% of liver enzyme type B remains | Homo sapiens | |
| p-chloromercuriphenylsulfonate | with 1 mM 38.3% of liver enzyme type S, 14.2% of brain enzyme type S, 1.9-2.2% of liver enzyme type A and 2.6-9.9% of liver enzyme type B remains | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | 0.4 | 4-methylumbelliferyl beta-N-acetylgalactosaminide | liver and brain enzymes type S | Homo sapiens | |
| 0.8 | 0.9 | 4-methylumbelliferyl-beta-N-acetylglucosaminide | liver and brain enzymes type S | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 152000 | - |
brain enzyme type S, gel filtration | Homo sapiens |
| 154000 | - |
liver enzyme type S, gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Homo sapiens | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
patient with Sandhoffs disease | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| liver enzyme type S 13500fold, brain enzyme type S 5240fold, liver enzymes types A and B | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.123 | - |
substrate: 4-methylumbelliferyl-beta-N-acetylglucosaminide, brain enzyme type S | Homo sapiens |
| 0.447 | - |
substrate: 4-methylumbelliferyl-beta-N-acetylglucosaminide, liver enzyme type S | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, liver enzyme type S, concentrated by ultrafiltration to 0.18 mg/ml, in 0.04 citrate buffer and in presence of 0.2 mg/ml bovine serum albumin stable for at least 3 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-N-acetylgalactosaminide + H2O | - |
Homo sapiens | 4-methylumbelliferone + beta-N-acetylgalactosamine | - |
? | |
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside | - |
? | |
| 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Homo sapiens | N-acetyl-D-hexosamines | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified brain enzyme, for 50 min, loses about 70% of activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
substrate: 4-methylumbelliferyl-beta-N-acetylglucosaminide or -acetylgalactosaminide, liver and brain enzymes type S | Homo sapiens |
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