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Literature summary for 3.2.1.52 extracted from
- Purification and characterization of a beta-N-acetylhexosaminidase of sea-squirt (1982), J. Biochem., 92, 163-172.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | with 7 mM 5% of activity remains | Styela plicata |  |
| Fe2+ | with 7 mM 16% of activity remains | Styela plicata | |
| Hg2+ | concentration of 7 mM results in total loss of activity | Styela plicata | |
| Monoiodoacetic acid | with 1 mM 73% of activity remains | Styela plicata | |
| p-chloromercuribenzoate | with 1 mM 20% of activity remains | Styela plicata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
SDS-PAGE | Styela plicata |
| 132000 | - |
gel filtration | Styela plicata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-beta-D-hexosaminides + H2O | Styela plicata | - |
N-acetyl-D-hexosamines | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Styela plicata | - |
sea-squirt | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1560fold, fairly homogenous | Styela plicata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| viscus | - |
Styela plicata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Styela plicata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, in 10% glycerol, pH 5.0-6.0, no loss of activity in 4 months | Styela plicata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| asialodegalactosyl fetuin glycopeptide + H2O | - |
Styela plicata | N-acetylglucosamine + ? | - |
? | |
| asialodegalactosyl ovomucoid glycopeptide + H2O | - |
Styela plicata | N-acetylglucosamine + ? | - |
? | |
| defucosyl Gi-x glycopeptide + H2O | poor substrate | Styela plicata | N-acetylglucosamine + ? | - |
? | |
| large fragment of hyaluronic acid + H2O | prepared by hyaluronidase treatment | Styela plicata | N-acetylglucosamine + ? | - |
? | |
| N-acetyl-beta-D-hexosaminides + H2O | - |
Styela plicata | N-acetyl-D-hexosamines | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O | - |
Styela plicata | 2-acetamido-2-deoxy-D-galactose + p-nitrophenol | - |
? | |
| p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O | - |
Styela plicata | 2-acetamido-2-deoxy-D-glucose + p-nitrophenol | - |
? | |
| small fragment of hyaluronic acid + H2O | prepared by hyaluronidase treatment, poor substrate | Styela plicata | N-acetylglucosamine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 66000, SDS-PAGE | Styela plicata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside | Styela plicata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
unstable at pH 4.0, i.e. the enzyme' pH optimum | Styela plicata |
| 5 | 6 | stable | Styela plicata |
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