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Literature summary for 3.2.1.52 extracted from

  • Gers-Barlag, H.; Bartz, I.; Ruediger, H.
    beta-N-Acetylhexosaminidase from soybean (1988), Phytochemistry, 27, 3739-3741.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Glycine max
galactosamine competitive inhibitor, less potent than glucosamine Glycine max
galactose very poor Glycine max
glucosamine competitive inhibitor, more potent than galactosamine Glycine max
glucose very poor Glycine max
N-acetyl-D-galactosamine competitive inhibitor; more potent than N-acetyl-D-glucosamine Glycine max
N-acetyl-D-glucosamine competitive inhibitor; less potent than N-acetyl-D-galactosamine Glycine max
Zn2+
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
Glycine max
0.48
-
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
133000
-
gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Glycine max
-
N-acetyl-D-hexosamines
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-

Purification (Commentary)

Purification (Comment) Organism
113fold Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O
-
Glycine max N-acetyl-D-hexosamines
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
Glycine max 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
Glycine max 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside Glycine max
5
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Glycine max