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Literature summary for 3.2.1.51 extracted from

  • Shi, R.; Yang, S.Q.; Wang, N.N.; Yan, Q.J.; Yan, X.M.; Jiang, Z.Q.
    Synthesis of 2-fucosyllactose from apple pomace-derived xyloglucan oligosaccharides by an alpha-L-fucosidase from Pedobacter sp. CAU209 (2023), Appl. Microbiol. Biotechnol., 107, 3579-3591.
    View publication on PubMed
Search for more literature for 3.2.1.51

Activating Compound

Activating Compound Comment Organism Structure
Ba2+ 1 mM, enhances activity by 11% Pedobacter sp. CAU209
Co2+ 1 mM, enhances activity by 7% Pedobacter sp. CAU209
Cr3+ 1 mM, enhances activity by 9% Pedobacter sp. CAU209
DTT 1 mM, enhances activity by 7% Pedobacter sp. CAU209
Fe2+ 1 mM, enhances activity by 5% Pedobacter sp. CAU209
Fe3+ 1 mM, enhances activity by 6% Pedobacter sp. CAU209
Li+ 1 mM, enhances activity by 6% Pedobacter sp. CAU209
Mg2+ 1 mM, enhances activity by 6% Pedobacter sp. CAU209
Mn2+ 1 mM, enhances activity by 9% Pedobacter sp. CAU209
Na+ 1 mM, enhances activity by 6% Pedobacter sp. CAU209
Ni2+ 1 mM, enhances activity by 2% Pedobacter sp. CAU209
Triton X-100 1 mM, enhances activity by 27% Pedobacter sp. CAU209
Zn2+ 1 mM, enhances activity by 2% Pedobacter sp. CAU209

Application

Application Comment Organism
synthesis the enzyme mediates the fucosylation of lactose and provides an efficient enzymatic strategy to synthesize 2'-fucosyllactose either from artificial pNP-Fuc or natural apple pomace-derived XyG-oligos Pedobacter sp. CAU209

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pedobacter sp. CAU209

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 93% residual activity Pedobacter sp. CAU209
Ag+ 1 mM, complete loss of activity Pedobacter sp. CAU209
Cu2+ 1 mM, 5% residual activity Pedobacter sp. CAU209
K+ 1 mM, 94% residual activity Pedobacter sp. CAU209
SDS 1 mM, 5% residual activity Pedobacter sp. CAU209

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 4 4-nitrophenyl-alpha-L-fucopyranoside pH 5.5, 35°C Pedobacter sp. CAU209
58
-
 2'-fucosyllactose pH 5.5, 35°C Pedobacter sp. CAU209

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 SDS-PAGE Pedobacter sp. CAU209

Organism

Organism UniProt Comment Textmining
Pedobacter sp. CAU209 A0A7D3USC4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pedobacter sp. CAU209

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.8
-
 substrate: 4-nitrophenyl-alpha-L-fucopyranoside, pH 5.5, 35°C Pedobacter sp. CAU209

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fucosyllactose + H2O 39% compared to the activity with 4-nitrophenyl-alpha-L-fucopyranoside Pedobacter sp. CAU209 alpha-L-fucose + lactose
-
 ?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Pedobacter sp. CAU209 4-nitrophenol + alpha-L-fucose
-
 ?
additional information transglycosylation activity with 4-nitrophenyl-alpha-L-fucopyranoside and lactose to 4-nitrophenol and 2'-fucosyllactose Pedobacter sp. CAU209 ?
-
-

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Pedobacter sp. CAU209

Synonyms

Synonyms Comment Organism
FucB
-
 Pedobacter sp. CAU209

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Pedobacter sp. CAU209

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 20°C: about 80% of maximal activity, 40°C: about 70% of maximal activity Pedobacter sp. CAU209

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable below, half-life: 252 min Pedobacter sp. CAU209
40
-
 half-life: 44 min Pedobacter sp. CAU209
45
-
 half-life: 5.5 min Pedobacter sp. CAU209

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pedobacter sp. CAU209

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5: about 70% of maximal activity, pH 7.0: about 60% of maximal activity Pedobacter sp. CAU209

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 10 more than 80% of enzyme activity is retained after incubation in buffers from pH 3.5 to 10.0 for 0.5 h Pedobacter sp. CAU209