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Literature summary for 3.2.1.51 extracted from

  • Benesova, E.; Lipovova, P.; Krejzova, J.; Kovalova, T.; Buchtova, P.; Spiwok, V.; Kralova, B.
    alpha-L-Fucosidase isoenzyme iso2 from Paenibacillus thiaminolyticus (2015), BMC Biotechnol., 15, 36 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.51

Cloned(Commentary)

Cloned (Comment) Organism
expression of a His-tagged enzyme in Escherichia coli BL21 (DE3) Paenibacillus thiaminolyticus

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl alpha-L-fucopyranoside substrate inhibition at high concentrations Paenibacillus thiaminolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
 4-nitrophenyl alpha-L-fucopyranoside pH 7.0, 37°C Paenibacillus thiaminolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76800
-
 protein with polyhistidine tag, SDS-PAGE Paenibacillus thiaminolyticus

Organism

Organism UniProt Comment Textmining
Paenibacillus thiaminolyticus K0JCW6
-
-
Paenibacillus thiaminolyticus CCM 3599 K0JCW6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenibacillus thiaminolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-fucopyranoside + H2O the enzyme shows a low transglycosylation potential Paenibacillus thiaminolyticus 4-nitrophenol + alpha-L-fucopyranose
-
 ?
4-nitrophenyl alpha-L-fucopyranoside + H2O the enzyme shows a low transglycosylation potential Paenibacillus thiaminolyticus CCM 3599 4-nitrophenol + alpha-L-fucopyranose
-
 ?

Subunits

Subunits Comment Organism
? x * 76800, protein with polyhistidine tag, SDS-PAGE Paenibacillus thiaminolyticus

Synonyms

Synonyms Comment Organism
alpha-L-fucosidase iso2
-
 Paenibacillus thiaminolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus thiaminolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
111
-
 4-nitrophenyl alpha-L-fucopyranoside pH 7.0, 37°C Paenibacillus thiaminolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Paenibacillus thiaminolyticus

General Information

General Information Comment Organism
physiological function the enzyme is involved in metabolism of alpha-L-fucosylated molecules, compounds with a fundamental role in different life essential processes including immune response, fertilization Paenibacillus thiaminolyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
213
-
 4-nitrophenyl alpha-L-fucopyranoside pH 7.0, 37°C Paenibacillus thiaminolyticus