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Literature summary for 3.2.1.51 extracted from

  • Cobucci-Ponzano, B.; Mazzone, M.; Rossi, M.; Moracci, M.
    Probing the catalytically essential residues of the alpha-L-fucosidase from the hyperthermophilic archaeon Sulfolobus solfataricus (2005), Biochemistry, 44, 6331-6342.
    View publication on PubMed
Search for more literature for 3.2.1.51

Protein Variants

Protein Variants Comment Organism
D124G kcat/Km at 65°C and pH 6.3 is 3.7fold lower than the wild-type value Saccharolobus solfataricus
D146G kcat/Km at 65°C and pH 6.3 is 1.5fold lower than the wild-type value Saccharolobus solfataricus
E292G kcat/Km at 65°C and pH 6.3 is 310.6fold lower than the wild-type value Saccharolobus solfataricus
E292G kcat/Km at 65°C and pH 6.3 is 310.6fold lower than wild-type value Saccharolobus solfataricus
E58G kcat/Km at 65°C and pH 6.3 is 1897fold lower than the wild-type value Saccharolobus solfataricus
E58G kcat/Km is 10.8-3897fold lower than wild-type value depending on pH-value and buffer Saccharolobus solfataricus
H123G kcat/Km at 65°C and pH 6.3 is 1719.8fold lower than the wild-type value Saccharolobus solfataricus
H46G kcat/Km at 65°C and pH 6.3 is 410fold lower than the wild-type value Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, wild-type enzyme Saccharolobus solfataricus
0.033
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme D146G Saccharolobus solfataricus
0.056
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme E292G Saccharolobus solfataricus
0.088
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme D124G Saccharolobus solfataricus
0.26
-
 4-nitrophenyl-alpha-L-fucopyranoside pH5.0, 65°C, wild-type enzyme Saccharolobus solfataricus
4.37
-
 4-nitrophenyl-alpha-L-fucopyranoside pH5.0, 65°C, mutant enzyme H46G Saccharolobus solfataricus
17
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme H46G Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + alpha-L-fucose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.96
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme E292G Saccharolobus solfataricus
224
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme D146G Saccharolobus solfataricus
240
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme D124G Saccharolobus solfataricus
287
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, wild-type enzyme Saccharolobus solfataricus
419
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme E292G Saccharolobus solfataricus
419
-
 4-nitrophenyl-alpha-L-fucopyranoside pH 6.3, 65°C, mutant enzyme H46G Saccharolobus solfataricus
430
-
 4-nitrophenyl-alpha-L-fucopyranoside pH5.0, 65°C, wild-type enzyme Saccharolobus solfataricus
495
-
 4-nitrophenyl-alpha-L-fucopyranoside pH5.0, 65°C, mutant enzyme H46G Saccharolobus solfataricus