KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-methylumbelliferyl alpha-L-fucopyranoside | pH 7.0, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
236000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
sialoglycoprotein | the enzyme occurs in different isoforms depending on the number of terminal sialic acid residues present on the glycoprotein molecules | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
seminal plasma | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 6 weeks | Homo sapiens |
2-4°C, pH 5.5 in presence of 1% L-fucose, 77% activity after 6 weeks | Homo sapiens |
21°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 3 days | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-L-fucopyranoside | - |
Homo sapiens | 4-methylumbelliferone + alpha-L-fucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 56000-57000, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
100% activity after 6 h | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | a broad acidic optimum at pH 4.0-4.5 and a second neutral optimum at pH 6.4-7.4 | Homo sapiens |
6.4 | 7.4 | a broad acidic optimum at pH 4.0-4.5 and a second neutral optimum at pH 6.4-7.4 | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | isoelectric focusing indicates that the purified enzyme consists of multiple isoforms | - |
additional information |