Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.51 extracted from

  • Khunsook, S.; Alhadeff, J.A.; Bean, B.S.
    Purification and characterization of human seminal plasma alpha-L-fucosidase (2002), Mol. Hum. Reprod., 8, 221-227.
    View publication on PubMed
Search for more literature for 3.2.1.51

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 4-methylumbelliferyl alpha-L-fucopyranoside pH 7.0, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
236000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
sialoglycoprotein the enzyme occurs in different isoforms depending on the number of terminal sialic acid residues present on the glycoprotein molecules Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
seminal plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 6 weeks Homo sapiens
2-4°C, pH 5.5 in presence of 1% L-fucose, 77% activity after 6 weeks Homo sapiens
21°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 3 days Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-L-fucopyranoside
-
 Homo sapiens 4-methylumbelliferone + alpha-L-fucose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 56000-57000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 100% activity after 6 h Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5 a broad acidic optimum at pH 4.0-4.5 and a second neutral optimum at pH 6.4-7.4 Homo sapiens
6.4 7.4 a broad acidic optimum at pH 4.0-4.5 and a second neutral optimum at pH 6.4-7.4 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing indicates that the purified enzyme consists of multiple isoforms
-
 additional information