Literature summary for 3.2.1.51 extracted from

Tarling, C.A.; He, S.; Sulzenbacher, G.; Bignon, C.; Bourne, Y.; Henrissat, B.; Withers, S.G.
Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis (2003), J. Biol. Chem., 278, 47394-47399.

Search for more literature for 3.2.1.51

Activating Compound

Activating Compound	Comment	Organism	Structure
azide	increases the rate of substrate cleavage in depending of azide concentration	Thermotoga maritima
formate	increases the rate of substrate cleavage in depending of azide concentration	Thermotoga maritima
methanol	linear increase in enzyme-catalyzed cleavage of p-nitrophenyl alpha-L-fucoside and fucosyl fluoride	Thermotoga maritima
NaCl	small positive effect	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
D2224A	the mutant shows a greatly reduced activity compared with wild-type enzyme	Thermotoga maritima

Inhibitors

Inhibitors	Comment	Organism	Structure
p-nitrophenyl alpha-L-fucoside	substrate inhibition at high concentration	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.032	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 60°C	Thermotoga maritima
0.034	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 37°C	Thermotoga maritima
0.23	-	alpha-L-fucosyl fluoride	pH 5.0, 25°C	Thermotoga maritima
2.1	-	2-deoxy-2-fluoro-alpha-L-fucosyl fluoride	pH 5.0, 60°C	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the addition of calcium, magnesium, cobalt, manganese, nickel, and zinc at 10 mM concentrations does not increase enzymatic rates	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9WYE2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride + H2O	-	Thermotoga maritima	fluoride + 2-deoxy-2-fluoro-alpha-L-fucose	-	?
alpha-L-fucosyl fluoride + H2O	-	Thermotoga maritima	alpha-L-fucose + fluoride	-	?
alpha-L-fucosyl fluoride + H2O	-	Thermotoga maritima	fluoride + alpha-L-fucose	-	?
p-nitrophenyl alpha-L-fucoside + H2O	-	Thermotoga maritima	p-nitrophenol + alpha-L-fucose	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	the enzyme shows no loss of activity after 24 h	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.047	-	2-deoxy-2-fluoro-alpha-L-fucosyl fluoride	pH 5.0, 60°C	Thermotoga maritima
2.9	-	alpha-L-fucosyl fluoride	pH 5.0, 25°C	Thermotoga maritima
5.4	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 37°C	Thermotoga maritima
9.1	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 60°C	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.3	-	-	Thermotoga maritima

pH Range

pH Minimum	pH Maximum	Comment	Organism
3.7	5.5	less than 50% of maximal activity above and below	Thermotoga maritima

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	-	rapid inactivation	Thermotoga maritima

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.64	-	p-nitrophenyl alpha-L-fucoside	pH 5.0, 60°C	Thermotoga maritima

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Release 2023.1 (January 2023)