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Literature summary for 3.2.1.51 extracted from

  • Khunsook, S.; Bean, B.S.; McGowan, S.R.; Alhadeff, J.A.
    Purification and characterization of plasma membrane-associated human sperm alpha-L-fucosidase (2003), Biol. Reprod., 68, 709-716.
    View publication on PubMed
Search for more literature for 3.2.1.51

General Stability

General Stability Organism
In the presence of 0.015% Triton X-100 the frozen enzyme retains 100% activity after 3 months, in absence of 0.015% Triton X-100 the enzyme loses all activity after 2 weeks. In the presence or absence of 0.015% Triton X-100 and 2-4°C retains 100% activity after 2 weeks. Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 4-methylumbelliferyl alpha-L-fucopyranoside pH 7.0, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 x * 51000, SDS-PAGE Homo sapiens
86000
-
 major form Homo sapiens
230000
-
 minor form Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information treatment of enzyme with neuraminidase does not change its isoform profile Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
sperm plasma membrane associated Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, in the presence of 1% fucose and presence or absence of 0.015% Triton X-100, 100% activity after 2 weeks Homo sapiens
2-4°C, in the presence of 1% fucose and 0.015% Triton X-100, 100% activity after 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-L-fucose
-
 ?

Subunits

Subunits Comment Organism
? x * 51000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.3
-
 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing, possible minor isoform
-
 6
Homo sapiens isoelectric focusing, isoform I
-
 6.5
Homo sapiens isoelectric focusing, isoform II
-
 7.1