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Literature summary for 3.2.1.51 extracted from

  • Jain, R.S.; Binder, R.L.; Levy-Benshimol, A.; Buck, C.A.; Warren, L.
    Purification of alpha-L-fucosidase from various sources by affinity chromatography (1977), J. Chromatogr., 139, 283-290.
    View publication on PubMed
Search for more literature for 3.2.1.51

Inhibitors

Inhibitors Comment Organism Structure
4-aminophenyl 1-thio alpha-L-fucoside weak competitive inhibition, Ki: 1.25 mM Rattus sp.
alpha-L-fucose competitive inhibition, Ki: 0.285 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.277
-
 4-nitrophenyl-alpha-L-fucopyranoside
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Clostridium perfringens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gastric mucin + H2O Clostridium perfringens porcine alpha-L-fucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-
Limulus polyphemus
-
-
-
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, affinity chromatography Limulus polyphemus
to homogeneity, affinity chromatography Clostridium perfringens
to homogeneity, affinity chromatography Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Clostridium perfringens
-
epididymis
-
 Rattus sp.
-
serum
-
 Limulus polyphemus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.39
-
-
 Limulus polyphemus
2.61
-
-
 Clostridium perfringens
15.6
-
-
 Rattus sp.

Storage Stability

Storage Stability Organism
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months Limulus polyphemus
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months Clostridium perfringens
4°C, 0.2 M sodium citrate, pH 6.0, 20% polyethylene glycol, 2 months Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Limulus polyphemus 4-nitrophenol + alpha-L-fucose
-
 ?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Rattus sp. 4-nitrophenol + alpha-L-fucose
-
 ?
gastric mucin + H2O porcine Clostridium perfringens alpha-L-fucose + ?
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.25
-
 4-aminophenyl 1-thio alpha-L-fucoside weak competitive inhibition Rattus sp.