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Literature summary for 3.2.1.50 extracted from
- N-Acetyl alpha-D-glucosaminidase from the venom of African puff adder (Bitis arietans) (2001), J. Biochem. Mol. Toxicol., 15, 221-227.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-thio-beta-D-N-acetylglucosamine | competitive | Bitis arietans |  |
| colombin | active component of antivenom preparation from Aristolodia albida, competitive | Bitis arietans | |
| Hg2+ | noncompetitive | Bitis arietans | |
| N-acetylglucosamine | competitive | Bitis arietans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00055 | - |
p-nitrophenyl-1-thio-N-acetylglucosamine | - |
Bitis arietans | |
| 0.00065 | - |
chitobiose | - |
Bitis arietans | |
| 0.0008 | - |
chitotriose | - |
Bitis arietans | |
| 0.001 | - |
methylumbelliferyl-N-acetylglucosamine | - |
Bitis arietans | |
| 0.003 | - |
chitotetraose | - |
Bitis arietans | |
| 0.0035 | - |
methylumbelliferyl-N-acetylglucosamine-6-sulfate | - |
Bitis arietans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
2 * 51000, SDS-PAGE | Bitis arietans |
| 102000 | - |
gel filtration | Bitis arietans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bitis arietans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bitis arietans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Bitis arietans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bitis arietans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitobiose + H2O | - |
Bitis arietans | ? | - |
? | |
| chitotetraose + H2O | - |
Bitis arietans | ? | - |
? | |
| chitotriose + H2O | - |
Bitis arietans | ? | - |
? | |
| methylumbelliferyl-N-acetylglucosamine + H2O | - |
Bitis arietans | methylumbelliferone + N-acetylglucosamine | - |
? | |
| methylumbelliferyl-N-acetylglucosamine-6-sulfate + H2O | - |
Bitis arietans | methylumbelliferone + N-acetylglucosamine-6-sulfate | - |
? | |
| additional information | swollen chitin and oligosaccharide above (GlcNAc)4 are not hydrolyzed by the enzyme | Bitis arietans | ? | - |
? | |
| p-nitrophenyl-1-thio-N-acetylglucosamine + H2O | - |
Bitis arietans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 51000, SDS-PAGE | Bitis arietans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAGase | - |
Bitis arietans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bitis arietans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, complete inactivation | Bitis arietans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
hydrolysis of methylumbelliferyl-N-acetylglucosamine | Bitis arietans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
1-thio-beta-D-N-acetylglucosamine | - |
Bitis arietans | |
| 0.55 | - |
Hg2+ | - |
Bitis arietans | |
| 0.6 | - |
colombin | - |
Bitis arietans | |
| 8 | - |
N-acetylglucosamine | - |
Bitis arietans | |
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