General Stability | Organism |
---|---|
the purified preparation of the enzyme can be stored frozen for a month and retains 100% of its activity after thawing | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaN3 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.15 | - |
4-nitrophenyl N-acetyl-D-galactosamine | pH 5.2, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55600 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P17050 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
DLD-1 cell | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
the purified preparation of the enzyme can be stored frozen for a month and retains 100% of its activity after thawing | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N-acetyl-D-galactosamine + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-galactosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55600, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alpha-NaGalase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 68 | 37°C: about 50% of maximal activity, 68°C: about 50% of maximal activity | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
half-life: 60 min | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0258 | - |
4-nitrophenyl N-acetyl-D-galactosamine | pH 5.2, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | the enzyme is more than 50% active between pH 4.0 and pH 5.5, at pH 6, the enzyme retains almost 40% of activity | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | fucoidan reduces the expression of the enzyme in the DLD-1 cells | down |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
4-nitrophenyl N-acetyl-D-galactosamine | pH 5.2, 37°C | Homo sapiens |