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Literature summary for 3.2.1.49 extracted from

  • Gao, H.; Li, S.; Bao, G.; Zhang, X.; Li, H.; Wang, Y.; Tan, Y.; Ji, S.; Gong, F.
    Glucose buffer is suitable for blood group conversion with alpha-N acetylgalactosaminidase and alpha-galactosidase (2014), Blood Transfus., 12, 61-66.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.49

Application

Application Comment Organism
diagnostics the enzyme is used for blood group conversion together with alpha-galactosidase, best in glucose buffer Elizabethkingia meningoseptica

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Elizabethkingia meningoseptica

Organism

Organism UniProt Comment Textmining
Elizabethkingia meningoseptica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Elizabethkingia meningoseptica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-galactosamine + H2O
-
 Elizabethkingia meningoseptica 4-nitrophenol + N-acetyl-beta-galactosamine
-
 ?

Synonyms

Synonyms Comment Organism
alpha-N acetylgalactosaminidase
-
 Elizabethkingia meningoseptica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
 assay at Elizabethkingia meningoseptica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Elizabethkingia meningoseptica

General Information

General Information Comment Organism
additional information comparison of the binding ability of alpha-N-acetylgalactosaminidase with red blood cells in different reaction buffers, such as normal saline, phosphate-buffered saline, a disodium hydrogen phosphate-based buffer, and 5% commercial glucose solution. Glucose buffer is suitable for blood group conversion with alpha-N acetylgalactosaminidase Elizabethkingia meningoseptica