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Literature summary for 3.2.1.49 extracted from
- Alpha-N-acetylgalactosaminidase from marine bacterium Arenibacter latericius KMM 426T removing blood type specificity of A-erythrocytes (2002), Biochemistry, 67, 689-695.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | The enzyme is able to remove at neutral pH alpha-1,3-bound residues of N-acetylgalactosamine from glycoproteins of blood group A-substances and A-erythrocytes, converting them into 0 group substances | Arenibacter latericius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dianisidine | 2 mM, 70% of original activity | Arenibacter latericius |  |
| Acetylimidazole | 5 mM, 30% of original activity | Arenibacter latericius | |
| Ca2+ | 1 mM, complete loss of activity | Arenibacter latericius | |
| carbodiimide | 1.8 mM, complete loss of activity | Arenibacter latericius | |
| diethylpyrocarbonate | 2 mM, 60% of original activity | Arenibacter latericius | |
| N-bromosuccinimide | 1.8 mM, complete loss of activity | Arenibacter latericius | |
| N-ethylmaleimide | 1.8 mM, 60% of original activity | Arenibacter latericius | |
| p-chloromercuribenzoate | 1.8 mM, complete loss of activity | Arenibacter latericius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
p-nitrophenyl-alpha-N-acetyl-D-galactosaminide | pH 7.2, 36°C | Arenibacter latericius | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Arenibacter latericius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
gel filtration | Arenibacter latericius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arenibacter latericius | - |
KMM 426 | - |
| Arenibacter latericius KMM 426 | - |
KMM 426 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arenibacter latericius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Arenibacter latericius |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, stable for 1 week | Arenibacter latericius |
| 4°C, sterile condition, stable for any length of time | Arenibacter latericius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-N-acetyl-D-galactosaminide + H2O | - |
Arenibacter latericius | p-nitrophenol + alpha-N-acetyl-D-galactosamine | - |
? | |
| p-nitrophenyl-alpha-N-acetyl-D-galactosaminide + H2O | - |
Arenibacter latericius KMM 426 | p-nitrophenol + alpha-N-acetyl-D-galactosamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-N-galactosaminidase IV | - |
Arenibacter latericius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme does not withstand freezing | Arenibacter latericius |
| 50 | - |
at heating during 30 min retains 100% of the activity | Arenibacter latericius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | pH 7.2, 36°C | Arenibacter latericius |
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Release 2023.1 (January 2023)
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