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Literature summary for 3.2.1.49 extracted from
- Purification and characterization of alpha-N-acetylgalactosaminidase from skipjack liver (1987), J. Biochem., 101, 855-862.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
ovine submaxillary asialoglycoprotein | - |
Katsuwonus pelamis | |
| 4.2 | - |
4-nitrophenyl alpha-N-acetylgalactosaminide | - |
Katsuwonus pelamis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, monomers associate to dimers at pH 4.2, SDS-PAGE | Katsuwonus pelamis |
| 40000 | - |
pH 7.2, gel filtration | Katsuwonus pelamis |
| 80000 | - |
pH 4.2, gel filtration | Katsuwonus pelamis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Katsuwonus pelamis | - |
skipjack tuna | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Katsuwonus pelamis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Katsuwonus pelamis | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, lyophilized, several years | Katsuwonus pelamis |
| 0°C, sodium citrate buffer, bovine serum albumin, 2 weeks, pH 4.2 | Katsuwonus pelamis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-N-acetylgalactosaminide + H2O | - |
Katsuwonus pelamis | 4-nitrophenol + alpha-N-acetylgalactosamine | - |
? |  |
| ovine submaxillary asialoglycoprotein + H2O | - |
Katsuwonus pelamis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, monomers associate to dimers at pH 4.2, SDS-PAGE | Katsuwonus pelamis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Katsuwonus pelamis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
p-nitrophenyl-alpha-N-acetylgalactosaminide | Katsuwonus pelamis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | not active above, p-nitrophenyl-alpha-N-acetylgalactosaminide | Katsuwonus pelamis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Katsuwonus pelamis |
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