Crystallization (Comment) | Organism |
---|---|
purified enzyme in the resting state with an empty active site, X-ray diffraction structure determination and analysis at 1.9 A resolution | Xanthomonas campestris pv. campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0607 | - |
sucrose | - |
Xanthomonas campestris pv. campestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | Xanthomonas campestris pv. campestris | - |
alpha-D-glucose + D-fructose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris pv. campestris | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Xanthomonas campestris pv. campestris | alpha-D-glucose + D-fructose | - |
? | |
sucrose + H2O | substrate binding site and structure, overview | Xanthomonas campestris pv. campestris | alpha-D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | residues Arg516 and Asp138 are not engaged in salt-bridge formation in the resting enzyme from Xanthomonas campestris pv. campestris in contrast to the enzyme from Xanthomonas axonopodis pv. glycines. In the absence of the salt bridge an opening is created which gives access to subsite 1 from the nonreducing end | Xanthomonas campestris pv. campestris |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the subfamily 4 of the glycoside hydrolase family 13, GH-13 | Xanthomonas campestris pv. campestris |
sucrose hydrolase | - |
Xanthomonas campestris pv. campestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.7 | - |
sucrose | - |
Xanthomonas campestris pv. campestris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 8.6 | - |
Xanthomonas campestris pv. campestris |