Application | Comment | Organism |
---|---|---|
medicine | findings could lead to an improved therapy for globoid cell leukodystrophy, GLD, or Krabbe disease | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression vectors for different fusion-proteins are constructed: GALC, unmodified enzyme, GALC-MH: fusion protein containing a C-terminal myc-tag and six His residues, GALC-TMH: fusion protein containing in addition a Tat-PTD, the protein transduction domain derived from the HIV-1 Tat protein | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
GALCdelta-MH | mutant containing a C-terminal myc-tag and six His residues with the last 11 amino acids at the C-terminus of GALC deleted | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Mus musculus | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
determined by SDS-PAGE and Western Blot analysis | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactosyl-N-acylsphingosine + H2O | Mus musculus | - |
D-galactose + N-acylsphingosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
myelin sheath | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
distribution of recombinant GALC activity in COS-7 cells: GALC-MH, 41.2% activity in cell extract, 58.8% released into cell culture medium, GALC-TMH, 27.5% to 72.5% | Mus musculus |
70 | - |
with plasmid encoding GALC-TMH transfected 293T cell, GALC units per mg of cell extract | Mus musculus |
120 | - |
with plasmid encoding GALC-MH transfected 293T cell, GALC units per mg of cell extract | Mus musculus |
130 | - |
with plasmid encoding unmodified GALC transfected 293T cell, GALC units per mg of cell extract | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactosyl-N-acylsphingosine + H2O | - |
Mus musculus | D-galactose + N-acylsphingosine | - |
? | |
psychosine + H2O | galactosylsphingosine | Mus musculus | D-galactose + sphingosine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
galactocerebrosidase | - |
Mus musculus |
Galactosylceramidase | - |
Mus musculus |
GALC | - |
Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
- |
Mus musculus |