Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heat-stable factor | - |
Homo sapiens |
General Stability | Organism |
---|---|
dithiothreitol stabilizes in later stages of chromatographic separation | Homo sapiens |
glycerol stabilizes in later stages of chromatographic separation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
beta-D-glucocerebroside | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucocerebroside + H2O | Homo sapiens | - |
glucose + ceramide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.6 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | - |
Homo sapiens | methylumbelliferone + glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Homo sapiens | 4-nitrophenol + D-glucopyranose | - |
? | |
glucocerebroside + H2O | - |
Homo sapiens | glucose + ceramide | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 60000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
addition of heat-stable factor shifts pH-optimum for hydrolysis of methylumbelliferyl-beta-D-glucose from 6.5 to 5.0 | Homo sapiens |
6 | 6.6 | - |
Homo sapiens |